Fig. 2
From: Calcium binding protects E-cadherin from cleavage by Helicobacter pylori HtrA
HtrA-mediated casein cleavage is independent of calcium ions. a 10 µg casein, composed of αS1-, αS2- and β-casein, was incubated with 200 ng HpHtrA and increasing concentrations of CaCl2 ranging from 25 to 400 µM as indicated. b Casein and HpHtrA were incubated with increasing concentrations of EDTA ranging from 25 to 400 µM. After 4 h incubation at 37 °C, samples were separated by SDS PAGE and proteins were stained with Coomassie G250. The original images of Coomassie-stained SDS gels are shown in Additional file 1: Figure S2