Fig. 6
From: Calcium binding protects E-cadherin from cleavage by Helicobacter pylori HtrA
EGTA enhances E-cadherin shedding of H. pylori-infected NCI-N87 cells. NCI-N87 cells were infected with H. pylori at a MOI of 100 (+) for 16 h or left untreated (−). Cells were treated with increasing concentrations of (a) CaCl2 or (b) EGTA. E-cadherin fragments (Cdh1NTF) in the supernatant and full length E-cadherin (Cdh1FL) in whole cell lysates were detected by western blotting. β-actin was detected as a control