Fig. 3From: Helicobacter pylori vacA s1m1 genotype but not cagA or babA2 increase the risk of ulcer and gastric cancer in patients from Southern Mexico H. pylori detection in DNA from biopsies of patients with gastric pathology and genotyping of vacA and status of cagA and babA2. a PCR amplification product of 16S rRNA gene in chronic gastritis patients. Lane 1 1 kb plus molecular weight marker; lane 2 positive control (DNA from H. pylori 26695 strain); lanes 3, 6, 7 negative samples; lanes 4, 5 positive samples; lane 8 negative control (without DNA). b PCR amplification product of 16S rRNA gene in gastric ulcer patients. Lane 1 1 kb plus molecular weight marker; lane 2 negative control (without DNA); lane 3 positive control (DNA from H. pylori 26695 strain); lanes 4, 8 positive samples; lanes 5–7 negative samples. c PCR amplification product of 16S rRNA gene in gastric cancer patients. Lane 1 1 kb plus molecular weight marker; lane 2 negative control (without DNA); lane 3 positive control (DNA from H. pylori 26695 strain); lanes 4, 5 negative samples; lanes 6–8 positive samples. d vacA genotypes. Lane 1 plus molecular weight marker 123 bp; lane 2 negative control (without DNA); lanes 3, 4 positive control (DNA from H. pylori ATCC 43504 strain vacA s1m1 genotype); lanes 5, 6 DNA from gastric biopsy with H. pylori vacA s1m1; lanes 7, 8 DNA from gastric biopsy with H. pylori vacA s2m2. e PCR amplification product of cagA gene. Lane 1 1 kb plus molecular weight marker; lane 2 negative control (without DNA); lane 3 positive control (DNA from H. pylori J99 strain cagA-positive); lanes 4, 5 clinical samples with H. pylori cagA-positive, lanes 6-8 clinical samples H. pylori cagA-negative. f PCR amplification product of babA2 gene. Lane 1 1 kb plus molecular weight marker; lane 2 negative control (without DNA), lane 3 positive control (DNA from H. pylori J99 strain babA2-positive); lanes 4, 5 clinical samples H. pylori babA2-positive; lanes 6–8 clinical samples H. pylori babA2-negativeBack to article page