Comparison of clinical and immunological findings in gnotobiotic piglets infected with Escherichia coli O104:H4 outbreak strain and EHEC O157:H7

Table 2 Antibody panels used for immunophenotyping by flow cytometry

Cell population addressed	Antigen	Clone	Isotype	Fluorochrome	Labelling strategy	Source of primary Ab
Myeloid cells, B cells and CD4⁺ T cells	CD3	PPT3	IgG1	BV 421^a	Biotin–streptavidin	In-house
Myeloid cells, B cells and CD4⁺ T cells	CD79α	HM57	IgG1	PE	Direct conjugation	Dako
	CD172α	74-22-15	IgG1	Alexa Fluor 647^b	Direct conjugation	In-house
	CD4	74-12-4	IgG2b	Alexa 488^c	Secondary antibody	In-house
T cells and NK-cell subpopulations	CD3	PPT3	IgG1	BV 421^a	Biotin–streptavidin	In-house
	CD4	74-12-4	IgG2b	Alexa Fluor 488^c	Secondary antibody	In-house
	CD8α	11/295/33	IgG2a	PE^d	Secondary antibody	In-house
	NKp46	VIV-KM1	IgG1	Alexa Fluor 647^b	Direct conjugation	In-house
γδ T-cell subpopulations	TCR-γδ	PPT16	IgG2b	BV421^a	Biotin–streptavidin	In-house
	CD8α	11/295/33	IgG2a	Alexa Fluor 647^b	Direct conjugation	In-house
	CD27	b30c7	IgG1	PE^e	Secondary antibody	In-house
	CD2	MSA4	IgG2a	Alexa Fluor 488^f	Direct conjugation	In-house

ISSN: 1757-4749