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Table 2 Antibody panels used for immunophenotyping by flow cytometry

From: Comparison of clinical and immunological findings in gnotobiotic piglets infected with Escherichia coli O104:H4 outbreak strain and EHEC O157:H7

Cell population addressed Antigen Clone Isotype Fluorochrome Labelling strategy Source of primary Ab
Myeloid cells, B cells and CD4+ T cells CD3 PPT3 IgG1 BV 421a Biotin–streptavidin In-house
CD79α HM57 IgG1 PE Direct conjugation Dako
  CD172α 74-22-15 IgG1 Alexa Fluor 647b Direct conjugation In-house
CD4 74-12-4 IgG2b Alexa 488c Secondary antibody In-house
T cells and NK-cell subpopulations CD3 PPT3 IgG1 BV 421a Biotin–streptavidin In-house
CD4 74-12-4 IgG2b Alexa Fluor 488c Secondary antibody In-house
CD8α 11/295/33 IgG2a PEd Secondary antibody In-house
NKp46 VIV-KM1 IgG1 Alexa Fluor 647b Direct conjugation In-house
γδ T-cell subpopulations TCR-γδ PPT16 IgG2b BV421a Biotin–streptavidin In-house
CD8α 11/295/33 IgG2a Alexa Fluor 647b Direct conjugation In-house
CD27 b30c7 IgG1 PEe Secondary antibody In-house
CD2 MSA4 IgG2a Alexa Fluor 488f Direct conjugation In-house
  1. aBrilliant Violet 421 streptavidin, BioLegend, San Diego, CA, USA
  2. bAlexa Fluor 647 Protein Labelling Kit, Invitrogen, Carlsbad, CA, USA
  3. cGoat anti-Mouse IgG2b-Alexa Fluor 488, Invitrogen, Carlsbad, CA, USA
  4. dGoat anti-Mouse IgG2a-PE, Southern Biotech, Birmingham, AL, USA
  5. eGoat anti-Mouse IgG1-PE, Southern Biotech, Birmingham, AL, USA
  6. fAlexa Fluor 488 Protein Labelling Kit, Invitrogen, Carlsbad, CA, USA