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Fig. 3 | Gut Pathogens

Fig. 3

From: Overexpression of serine protease HtrA enhances disruption of adherens junctions, paracellular transmigration and type IV secretion of CagA by Helicobacter pylori

Fig. 3

Elevated HtrA secretion levels in H. pylori does not affect the extracellular delivery of VacA and GGT. a H. pylori wild-type strain 26695 (Hp wt) and 26695 transformed with htrA 26695 were grown for 24 h in BHI broth medium with 1% β-cyclodextrin in the presence or absence of 1 or 2 mM IPTG, respectively. Bacteria-free supernatants were prepared and subjected to Western blotting using α-HtrA, α-VacA and α-GGT antibodies. The blots against α-CagA served as control. The band intensities of secreted HtrA (b) as well as VacA and GGT (c) were quantified densitometrically. The relative protein expression is given in “fold change”. All secretion assays were done in triplicates

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Gut Pathogens

ISSN: 1757-4749