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Fig. 7 | Gut Pathogens

Fig. 7

From: Overexpression of serine protease HtrA enhances disruption of adherens junctions, paracellular transmigration and type IV secretion of CagA by Helicobacter pylori

Fig. 7

Elevated E-cadherin cleavage and CagA translocation in host cells by overexpression of HtrA. Polarized MKN-28 cells were infected with IPTG-induced or control Hp wt or Hp htrA 26695 for 24 h. a Cell pellets and supernatants were prepared and subjected to α-E-cadherin Western blotting. The full-length E-cadherin signals (130 kDa) in the cell pellets and cleaved-off NTF-domain (90 kDa) in the culture supernatants are marked with arrows. b Translocation and phosphorylation of CagA (arrow) were analyzed using α-PY-99 and α-CagA antibodies, respectively. The asterisk marks a phosphorylated 125 kDa host cell protein migrating below CagA. c The band intensities of cleaved E-cadherin NTF-fragments and phospho-CagA signals were quantified densitometrically. The relative amounts of NTF-fragment and phosphorylated CagA are given in “fold change”. All experiments were done in triplicates

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Gut Pathogens

ISSN: 1757-4749