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Fig. 5 | Gut Pathogens

Fig. 5

From: Toll-like receptor-4 differentially mediates intestinal and extra-intestinal immune responses upon multi-drug resistant Pseudomonas aeruginosa association of IL10−/− mice with chronic colitis

Fig. 5

Histopathological mucosal changes, apoptotic and proliferating epithelial cells in large intestines of IL10−/− mice lacking TLR4 following peroral association with multi-drug resistant P. aeruginosa. Conventionally colonized IL10 deficient (WT IL10−/−; closed circles) and TLR4 deficient IL10−/− mice (TLR4−/− × IL10−/−; closed triangles) were perorally challenged with a clinical multi-drug resistant P. aeruginosa (PA) strain at day (d) 0. Fourteen days thereafter a histopathological mucosal changes were assessed in hematoxylin and eosin stained colonic paraffin sections applying a standardized histopathological scoring system. In addition, the average numbers of colonic epithelial, b apoptotic (positive for caspase 3, Casp3) and c proliferating cells (positive for Ki67) were determined from six high power fields (HPF, 400× magnification) per animal in immunohistochemically stained large intestinal paraffin sections. Naive (N) mice served as negative controls (open symbols). Numbers of mice (in parentheses), median (black bars) and significance levels (p values) determined by the Mann–Whitney U test are indicated. Data shown were pooled from three independent experiments

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Gut Pathogens

ISSN: 1757-4749