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Table 1 List of strains and plasmids used in this study

From: N-Acetyl-glucosamine influences the biofilm formation of Escherichia coli

Strains Characteristics Origin Disease References
LF82 Wild type AIEC O83:H1 AmpR Human Crohn’s disease [4]
LF82ΔnagC LF82 ΔnagC AmpR KanR    This study
MG1655 Laboratory E. coli K-12 Human Healthy [47]
EDL933 Wild type EHEC O157:H7 Human Diarrhea, HUS [48]
17.2 Wild type EAEC Human Diarrhea [49]
NC101 Wild type commensal (AIEC-like) Mouse Mouse colitis [50]
χ7213 thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpir ΔasdA4 Δzhf-2::Tn10    [51]
Plamids Characteristics References   
pGEM®-T Cloning vector Promega Corp., Madison, WI, USA   
pMEG-375 Suicide vector sacRB mobRP4 oriR6K; CmR ApR Roy Curtiss III, Arizona State University, Tempe, AZ, USA   
nagC pMEG-375 with ΔnagC:: KmR [16]   
pTrc99a expression vector with IPTG inducible lacI promoter; ApR [52]   
pnagC pTrc99a with nagC [16]   
pnagC-JFS pnagC with CmR This study