From: N-Acetyl-glucosamine influences the biofilm formation of Escherichia coli
Strains | Characteristics | Origin | Disease | References |
---|---|---|---|---|
LF82 | Wild type AIEC O83:H1 AmpR | Human | Crohn’s disease | [4] |
LF82ΔnagC | LF82 ΔnagC AmpR KanR | This study | ||
MG1655 | Laboratory E. coli K-12 | Human | Healthy | [47] |
EDL933 | Wild type EHEC O157:H7 | Human | Diarrhea, HUS | [48] |
17.2 | Wild type EAEC | Human | Diarrhea | [49] |
NC101 | Wild type commensal (AIEC-like) | Mouse | Mouse colitis | [50] |
χ7213 | thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpir ΔasdA4 Δzhf-2::Tn10 | [51] |
Plamids | Characteristics | References | ||
---|---|---|---|---|
pGEM®-T | Cloning vector | Promega Corp., Madison, WI, USA | ||
pMEG-375 | Suicide vector sacRB mobRP4 oriR6K; CmR ApR | Roy Curtiss III, Arizona State University, Tempe, AZ, USA | ||
pΔnagC | pMEG-375 with ΔnagC:: KmR | [16] | ||
pTrc99a | expression vector with IPTG inducible lacI promoter; ApR | [52] | ||
pnagC | pTrc99a with nagC | [16] | ||
pnagC-JFS | pnagC with CmR | This study |