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Fig. 2 | Gut Pathogens

Fig. 2

From: Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai

Fig. 2

Optimized DP-HMGS maintained high sensitivity for the simultaneous detection of all specific viral and bacterial pathogens. The detection limits of the DP-HMGS assay were determined by amplifying tenfold diluted plasmids containing equal amounts of 17 pathogen and 3 quality control templates at a 1 × 105, b 1 × 104, and c 1 × 103 copies/μL in the DP-HMGS assay. d ddH2O was used as the negative control. Note that the pathogen-defining DNA targets (from left to right: S. typhimurium, S. enteritidis, EIEC, Shigella, HADV, EHEC, HASV, Y. enterocolitica, ETEC, Vibrio, C. difficile, C. jejuni, E. coli O157, norovirus, EPEC, EAEC, rotavirus) all generated specific peaks. The quality controls Hum_RNA, Hum_DNA and IC produced specific peaks at 106 bp, 233 bp and 313 bp, respectively

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Gut Pathogens

ISSN: 1757-4749