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Fig. 3 | Gut Pathogens

Fig. 3

From: Clinical application of a multiplex genetic pathogen detection system remaps the aetiology of diarrhoeal infections in Shanghai

Fig. 3

DP-HMGS robustly detected multiple DP signatures without interference between multiple signatures. a The plasmids of S. enteritidis, HADV and EHEC combined at different proportions (1 × 103 copies, 1 × 105 copies, and 1 × 104 copies, respectively) produced specific peaks at 120 bp, 145 bp and 152 bp with low, high and midrange signal intensities (2600 rfu, 20,000 rfu and 6000 rfu, respectively). b The combined plasmids of three DPs with 7 negative control pathogen templates consistently showed the specific peaks of S. enteritidis, HADV and EHEC with no interference. Notably, the 313-bp peak for the IC was generated in each DP-HMGS reaction. The individually tested S. enteritidis (c), HADV (d), and EHEC (e) produced specific peaks at 120 bp, 145 bp, and 152 bp, respectively, with corresponding intensities of 2600 rfu, 20,000 rfu and 6000 rfu. Notably, the 313-bp peak for the IC was generated in each DP-HMGS reaction

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Gut Pathogens

ISSN: 1757-4749