Fig. 3

Enlarged images of gut epithelial cells from biopsies of non-infected and C. jejuni-infected patients. Confocal laser-scanning microscopy images at high magnification show in detail colon crypts from a healthy patient (a) and patient infected with C. jejuni (b). The biopsies are stained against occludin (red), ZO-1 (green) and the DNA with DAPI (blue). The overlay images (bottom, right) show the co-localization from occludin and ZO-1 (yellow) in the non-infected control (a) and in a disturbed tight junction pattern in the infected sample (b). Blue arrows indicate the apical labelling of occludin in non-infected controls, while white arrows indicate cytosolic occludin signals in the infected samples