Fig. 2

1,25(OH)₂D₃ deficiency alters the composition of the gut microbiota in the Cyp27b1^−/− mice. a Abundance distribution of TOP 10 bacterial taxa for 8–10-month wild type and Cyp27b1^−/− mice at class level. N = 5. b Student’s t test analysis revealed the significant changed bacteria in species including Akkermansia muciniphila (A. muciniphila), Bacteroides-acidifaciens, Bacteroides plebeius, Bacteroides uniformis, Roseburia inulinivorans, and Solitalea canadensis in Cyp27b1^−/− mice. c Relative abundance of A. muciniphila in feces of 8–10-month wild type and Cyp27b1^−/− mice. d Gas chromatography was performed to measure the concentration of butyrate in feces from 8 to 10-month wild type and Cyp27b1^−/− mice. *P < 0.05, **P < 0.01. WT: wild type, KO: Cyp27b1^−/−