Fig. 1From: RETRACTED ARTICLE: EV71 virus reduces Nrf2 activation to promote production of reactive oxygen species in infected cellsEV71 infection induces ROS generation, apoptosis, and inflammation of infected cells. a–c Reinforced mRNA and protein of VP1 in EV71-infected Vero and RD cells was identified in comparison to non-infected cells (Control) using western blotting and qPCR analyses. d ROS generation was examined in infected Vero and RD cells as evidenced by DCF fluorescence intensity assay compared to non-infected normal mice (Control). e, f Annexin V-FITC and PI flow cytometry was performed to assess the number of apoptotic Vero and RD cells. The upper right quadrant of every plot represents early dead cells. g qPCR analyses of the inflammation-promoting cytokines IL-1β, IL-6, and TNFα produced by infected cells. h The protein expression levels of IL-1β, IL-6, and TNFα of the infected cell were quantified using ELISA. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control groupBack to article page