Skip to main content
Fig. 3 | Gut Pathogens

Fig. 3

From: EV71 virus reduces Nrf2 activation to promote production of reactive oxygen species in infected cells

Fig. 3

Keap1 silencing upregulates Nrf2 activation in EV71-infected cells. Vero and RD cells were infected by EV71 at an MOI of 5 subsequent to 24 h transfection with Keap1 siRNA vector. a, b Western blot examination of the expression of Keap1, Nrf2, and HO-1 proteins in infected and non-infected cells. c, d Nrf2 was initially precipitated and western blot was performed to assess Nrf2 and ubiquitin. eg qPCR was used to assess the mRNA of Keap1, Nrf2, and HO-1 in infected and non-infected cells. h, i Immunofluorescence was utilized to assess cytoplasmic as well as nuclear location of Nrf2 (tetramethylrhodamine, red). Nuclear DNA appears blue due to DAPI. Data are presented as mean ± SD. *P < 0.05, **P < 0.01 vs. Control group

Back to article page