Fig. 4
From: Host responses to Clostridium perfringens challenge in a chicken model of chronic stress
Sialic acid- and sulfate-containing O-glycans are inversely correlated, but Clostridium perfringens challenge and stress do not significantly affect total sialic acid levels. Birds were administered 0.2% ethanol drinking water (Cp−St−), challenged with 107 CFU C. perfringens (Cp+St−), administered 20 mg/L CORT (Cp−St+), or received both C. perfringens and CORT challenge (Cp+St+). Treatments commenced in birds at 14-days-of-age, where C. perfringens was administered for 2 days and CORT for 7 days. a A Spearman’s rank correlation test was performed to determine significant associations between relative levels of all integrated O-glycans detected by CE (26) in all mucus samples (n = 16); x = ρcrit < 0.50 (P < 0.050) and X = ρcrit < 0.64 (P = 0.010). b Mucus samples were hydrolyzed (i), and labelled with 1,2-diamino-4,5-dimethylbenzene (DMBA; (ii) to permit sialic acid quantitation by HPLC–MS (iii). c No significant differences in sialic acid levels were observed among the treatments, although stress tended to lower levels (as a fraction of mucus mass) in both Cp−St+ and Cp+St+ birds. Differing letter codes above each treatment indicate significant differences by the adjusted P-values produced by the HSD test