Fig. 2

Boxplots of the melting temperatures (Tm) of Blastocystis spp. (red), Cryptosporidium spp. (brown), D. fragilis (green), E. histolytica (turquoise), Giardia intestinalis assemblage B (pink) and Giardia intestinalis assemblage A (blue). The box indicates the likely range of melting temperature variation. The melting temperature ranges were 71.5–71.5 °C for Dientamoeba, 75–75.5 °C for Entamoeba, 78–78.5 °C for Cryptosporidium, 79.5–80 °C for Blastocystis, 81.5–82 °C for Giardia assemblage B, and 83–83.5 °C for Giardia assemblage A. The melting temperature difference (∆Tm) between the closest melting curves were 3.7 °C (Dientamoeba/Entamoeba), 3.03 °C (Entamoeba/Cryptosporidium), 1.61 °C (Cryptosporidium/Blastocystis), 1.67 °C (Blastocystis/Giardia assemblage B) and 2 °C (Giardia assemblage B/A)