Fig. 4

Melting peaks of mixed infection samples presenting 4 cases of double infection and one case of triple infection. All qPCR runs were done with the 5 primers mix and one color is attributed to each parasite species and the samples (black and grey): Dientamoeba (brown)/Giardia B (pink) a, Entamoeba (green)/Blastocystis (Blue) b, Dientamoeba/Blastocystisc, Entamoeba/Blastocystis/Giardia assemblage A (red) d, and Blastocystis/Giardia assemblage A e. Singleplex runs were done with the target primers to confirm each mixedinfection