Fig. 2

CagA and eEF1A1 co-mediate the expression of IL-6. a The comparison of IL-6 RNA (1) and IL-6 (2) in AGS cells that infected with NCTC11637 ΔcagA or NCTC11637 bacterial inoculums (MOI = 100, 16 h). b Fluorescence microscope image and eEF1A1 level using western blotting (WB) analysis of AGS cells that transduced with LV-eEF1A1-RNA or LV-CON077-RNA for 48 h. c The levels of IL-6 in AGS-C and AGS-sheEF1A1 cells that infected with blank or NCTC11637 or NCTC11637ΔcagA. d Fluorescence microscope image of AGS cells transduced with Ad-cagA (CagA fusion virus particles) or Ad-GFP (a negative control) (1), CagA was detected by WB analysis (2); The levels of IL-6 in AGS-C and AGS-sheEF1A1 cells that transduced with blank or Ad-GFP or Ad-cagA treated by OSM (100 ng/mL, 24 h) or the drug vehicle (3). Error bar represents the SDs of triplicate experiments. Statistical analysis was performed using Student’s t-tests. *P < 0.05; **P < 0.01; ***P < 0.001