Table 1 Oligonucleotide primers used in this study
Target | Primers (forward/reverse, 5′-3′) |
|---|---|
Construction of mutants | |
 qsvR | GTGACTGCAGATGCTAAAAGCGGTGATTC/GATTCAAATGCGATTTCTGTTGGCTGGTGGACGACTAATG |
CATTAGTCGTCCACCAGCCAACAGAAATCGCATTTGAATC/GTGAGCATGCGAGAAGTCTGTAAACGAAACG | |
GTGACTGCAGATGCTAAAAGCGGTGATTC/GTGAGCATGCGAGAAGTCTGTAAACGAAACG | |
 toxR | GTGACTGCAGAAACGCAATTTGTCTGATG/ATCTTCATGCTGGCCTCCTTTAGTTCTTCTTAGATGGATGATG |
CATCATCCATCTAAGAAGAACTAAAGGAGGCCAGCATGAAGAT/GTGAGCATGCAATTCGGCGGCTTTGTTC | |
GTGACTGCAGAAACGCAATTTGTCTGATG/GTGAGCATGCAATTCGGCGGCTTTGTTC | |
Protein expression | |
 qsvR | AGCGGGATCCATGCCGAACATTGAGATCATTC/AGCGAAGCTTTTAACCTCTTACTACCTGATTACG |
qPCR | |
 toxR | TTGTTTGGCGTGAGCAAGG/TAGCAGAGGCGTCATTGTTATC |
 calR | ATGTAAAAAGAAAACCGTACA/AACACAGCAGAATGACCGTG |
 cpsQ | GCCTGAAATCCTAATGCTC/AGTGTCAGAAGGTGTATCAAC |
 mfpA | GCGGGCAATGATCGTCTAAC/TCACCTGAACCTGCGACAAG |
Primer extension | |
 toxR | /TTAGTTCTTCTTAGATGGATGATG |
 calR | /GCAAAATATCGGTACTTCA |
 cpsQ | /GATTTCAGGCTTTTCCGTGTAC |
 mfpA | /ATTCCCTCTGGCTTATTTATTG |
LacZ fusion | |
 toxR | GCGCGTCGACATCGTTAAGGTATTTGCA/GCGCGAATTCCGAGCGAATTACTATTTGG |
 calR | GCGGTCGACGTTTGTTTGCTCGGATTGTTTG/GCGTCTAGACAAAGTGCTTTCCATACGGTAG |
 cpsQ | GCGCGTCGACCAGACGGGCATTGATAAG/GCGCGAATTCCATTAGGATTTCAGGCTTTT |
 mfpA | GCGCGTCGACTTATGACTTAGATACCGAA/GCGCGAATTCCGAAATCAGCGATATTGTTG |
EMSA | |
 toxR | ATCGTTAAGGTATTTGCA/CGAGCGAATTACTATTTGG |
 calR | GTTTGTTTGCTCGGATTGTTTG/CAAAGTGCTTTCCATACGGTAG |
 cpsQ | GTTCCAGCAATACTGACTAAGC/GATTTCAGGCTTTTCCGTGTAC |
 mfpA | TAGGACGCAAGCCACAAG/CGAAATCAGCGATATTGTTG |
DNase I footprinting | |
 toxR | TTTCAGGGACGACTTTGTG/TTAGTTCTTCTTAGATGGATGATG |
 calR | ATTCCCTCTGGCTTATTTATTG/CCACGGCATTACTTACTG |
 cpsQ | TACCTAACTAATTTAGTGCA/GATTTCAGGCTTTTCCGTGTAC |
 mfpA | ACATACTATTAAATCGCATC/ATTCCCTCTGGCTTATTTATTG |