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Table 2 MAP: Blood ELISA, INFγ, plaque qPCR & fecal PCR

From: Comparison of a mycobacterial phage assay to detect viable Mycobacterium avium subspecies paratuberculosis with standard diagnostic modalities in cattle with naturally infected Johne disease

MAP Control Johne
Pos Neg Pos Neg
% # % # % # % #
ELISA 0 0/17 100 17/17 31 10/32 69 22/32
IFNγ 12 2/17 88 15/17 69 22/32 31 10/32
Fecal PCR 0 0/17 100 17/17 75 24/32 25 8/32
Phage / qPCR 35 6/17 65 11/17 72 23/32 28 9/32
  1. A summary of standard test results compared with the data from the phage/qPCR experiments. In the Control animals the ELISA and fecal PCR have no positives, whereas IFN-γ was 12% and phage / qPCR 35% positive. ELISA (31%+) is least sensitive in the Johne animals. IFN-γ (69%+), fecal PCR (75%+) and phage / qPCR (72%+) the most sensitive