Comparison of a mycobacterial phage assay to detect viable Mycobacterium avium subspecies paratuberculosis with standard diagnostic modalities in cattle with naturally infected Johne disease

Table 2 MAP: Blood ELISA, INFγ, plaque qPCR & fecal PCR

A summary of standard test results compared with the data from the phage/qPCR experiments. In the Control animals the ELISA and fecal PCR have no positives, whereas IFN-γ was 12% and phage / qPCR 35% positive. ELISA (31%+) is least sensitive in the Johne animals. IFN-γ (69%+), fecal PCR (75%+) and phage / qPCR (72%+) the most sensitive

ISSN: 1757-4749