Table 5 Primers used for PCR amplification of cagA, vacA, iceA, oipA and dupA genes
Gene | Primer | Primer sequence (5'–3') | Product size (bp) |
|---|---|---|---|
cagA | cagA3'-F | TGCGTGTGTGGCTGTTAGTAG | 593–752 |
cagA3'-R | CCTAGTCGGTAATGGGTTGT | ||
vacA | |||
s1/s2 | Vs-F | ATGGAAATACAACAAACACAC | 259/286 |
VA-R | CTGCTTGAATGCGCCAAAC | ||
s1a | Vs1a-F | GTCAGCATCACACCGCAAC | 190 |
VA-R | CTGCTTGAATGCGCCAAAC | ||
s1b | Vs1b-F | AGCGCCATACCGCAAGAG | 187 |
VA-R | CTGCTTGAATGCGCCAAAC | ||
s1c | Vs1c-F | CTCTCGCTTTAGTGGGGYT | 213 |
VA-R | CTGCTTGAATGCGCCAAAC | ||
m1 | Vm1-F | GGCCCCAATGCAGTCATGGAT | 240 |
Vm1-R | GCTGTTAGTGCCTAAAGAAGCAT | ||
m2 | Vm2-F | GGAGCCCCAGGAAACATTG | 352 |
Vm2-R | CATAACTAGCGCCTTGCAC | ||
i1 | Vi-F | GTTGGGATTGGGGGAATGCCG | 495 |
Vi1-R | TTAATTTAACGCTGTTTGAAG | ||
i2 | Vi-F | GTTGGGATTGGGGGAATGCCG | 495 |
Vi2-R | GATCAACGCTCTGATTTGA | ||
iceA1 | iceA1-F | GTGTTTTTAACCAAAGTATC | 246 |
iceA1-R | CTATAGCCAGTCTCTTTGCA | ||
iceA2 | iceA2-F | GTTGGGTATATCACAATTTAT | 229 |
iceA2-R | TTGCCCTATTTTCTAGTAGGT | ||
oipA | oipA-F | CGCGGAAAGGAACGGGTTTT | 519 |
oipA-R | TTAGCGTCTAGCGTTCTGCC | ||
dupA | dupA-F | GACGATTGAGCGATGGGAATAT | 971 |
dupA-R | CTGAGAAGCCTTATTATCTTGTTGG | ||