Fig. 1

The PBP1A sequence, structure in H. pylori and its interaction with AMX. a A segment of the pairwise sequence alignment of PBP1A in H. pylori and Streptococcus. The equivalent Ser368 and Ser370 in H. pylori and Streptococcus are shown in red. b The 3D structural model, transglycosylase, and transpeptidase domains are shown in silver, pink, and green cartoon secondary structures, respectively. The surface of the transpeptidase domain and the binding site are depicted in the inset figure. c The formation of the covalent interaction between the AMX and Ser368. The Ser368 and AMX are colored in red and blue, respectively. In the 2D panel, the non-covalent interaction between AMX-Ser368 and other PBP1A residues are presented. The protein residues involved in hydrophobic contacts and hydrogen bonds are represented in red spoked arcs and green dotted lines, respectively