Table 1 Bacterial strains and plasmids used in this study
Bacterial strains and plasmids | Characteristics | Purpose | Source |
|---|---|---|---|
Strains | |||
 E. coli M15 | F-, Φ80ΔlacM15, thi, lac-, mtl-, recA + , KmR | Recombinant protein expression (pQE30-hcp) | Qiagen, USA |
 E. coli DH5α | F-u80dlacZDM15 D (lacZYA- argF) U169 endA1 recA1 hsdR17(rk- mk) deoR thi-1 supE44 k-gyrA96 relA1 | Recombinant plasmid storage (cloning vector) | BioBharati, India |
 E. coli Top 10 | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG | Recombinant plasmid storage (L. lactis based plasmid constructs) | Thermo Fisher Scientific, USA |
 L. lactis NZ9000 | MG1363 (nisRK genes into chromosome), Wild type, plasmid free | Wild Type Control | Dr. L. G. Bermúdez Humarán, INRA, France |
 C. jejuni | Human clinical isolate (BCH 71) | For hcp gene cloning and infection study | Dr. Asish Kumar Mukhopadhyay, Scientist F, NICED (ICMR), Kolkata, India |
Plasmids | |||
 pQE30 | Ampr, His6 (N terminal), T5 promoter | Prokaryotic expression vector | Addgene, USA |
 pQE30-hcp | Ampr, pQE30 harboring hcp | Recombinant Hcp expression vector | [31] |
 pNZ8048-CWAM6 | Cmr, spUSP45, M6 cell wall anchor expressed under PnisA promoter | L. lactis based rHcp expression vector | Dr. L. G. Bermúdez Humarán, INRA, France |
 pNZ8048-CWAM6- hcp | Cmr, spUSP45, M6 cell wall anchor expressed under PnisA promoter | L. lactis based rHcp expression vector | This work |
 pSEC-hcp | Cmr, spUSP45 under PnisA promoter | L. lactis based rHcp expression vector | This work |