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Fig. 8 | Gut Pathogens

Fig. 8

From: Microbiome diversity declines while distinct expansions of Th17, iNKT, and dendritic cell subpopulations emerge after anastomosis surgery

Fig. 8

A new population of CD11bhi CD103mid DCs emerges after both end-to-end and Roux-en-Y anastomosis and are expressing IL-10. Intestinal mucosal cells were harvested 3 days post-operatively and stained extracellularly with CD45, IA-IE, CD110, CD11b, and CD103 and intracellularly with IL-10. Samples were then run on either a BD LSRII or Symphony. A Dot plot representation and B % of live cell numbers of CD11b+ CD103+ and C CD11bhi CD103mid DC (CD45+ IA-IE+ CD110+) populations. D Expression of IL-10 in CD11b CD103 DC populations. E Percent of live cell numbers of CD11bhi CD103mid DCs that are TGFβ+ *p  ≤  0.05–no surgery, n  =  6–9

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Gut Pathogens

ISSN: 1757-4749