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Table 3 Trail designs

From: Experimental induction of necrotic enteritis with or without predisposing factors using netB positive Clostridium perfringens strains

Trial

Na

Groups

Dietary factore

Coccidia challenge

C. perfringens challenge

Sampling day

1

24

G1(CP(FJ6) (C8-1))b

−

−

Day 14, 15, 16

Day 17(D1)g, 19(D3)h, 23

24

G2(E.n(G))c

−

Day 09

−

24

G3(CTL)d

−

−

−

2

24

G4(CP(FJ6) (C8-1))

+f

−

Day 15,16,17,18

Day 19 (D1), 21(D3), 23

24

G5(E.n(G))

+

Day 10

−

24

G6(CP(FJ6) (C8-1) + E.n(G))

+

Day 10

Day 15,16,17,18

24

G7(CTL)

+

−

−

  1. aN: number of chickens
  2. bCP(FJ6) and (C8-1): C. perfringens type G strain (FJ6), (C8-1)
  3. cE.n(G)Eimeria necatrix Guangdong strain
  4. dCTL: control group
  5. eDietary factor: maize-based diet containing 60% fishmeal (1:1)
  6. fDay 08 onward till the end of trial
  7. gD1: 1 day after the last challenge of C. perfringens type G strain
  8. hD3: 3 days after the last challenge of C. perfringens type G strain