Fig. 2

Curcumin slightly inhibits EV71 proliferation. SH-SY5Y cells were treated with 3.125 to 50 μM Curcumin for 0 to 36 h followed by cell viability assessment using MTT (a). 5 MOI of EV71 were pre-incubated with 5 μM or 20 μM Curcumin at 37 °C for 2 h, then the virus were used to infect SH-SY5Y cells with binding buffer on ice. 1 h later, the cells were washed (attachment assessment) or/and cultured at 37 °C for another 1 h and then treated with trypsin (internalization assessment). Viral RNA was extracted by commercial kit and EV71 RNA was determined by RT-qPCR (b). SH-SY5Y cells were pre-transfected with Cap-Rluc-vIRES-Fluc mRNA (100 ng/well, 96-well plate). 4 h later, the cells were infected with mock or EV71 for 2 h and then the cells were treated with 5 or 20 μM Curcumin for 10 h. Intensity of FLuc and RLuc were measured as Materials and Methods described and Fluc/Rluc indicates IRES activity (c). Progeny viruses in supernatant from Fig. 1c were titrated using RD cells and the results presented as log₁₀ TCID₅₀/mL (d). All the results indicate the mean ± SD of three independent experiments. Statistical analysis was performed using Student’s t-test, *P < 0.05, ** P < 0.01, n.s. P > 0.05. EV, EV71; C, Curcumin