Fig. 2
From: Invasion of intestinal cells by Staphylococcus warneri, a member of the human gut microbiota
S. warneri can invade Caco2 cells. A Description of the gentamicin/imipenem-protection assay used to quantify internalization efficiency in Caco2 cells. B Quantification of the intracellular bacteria, expressed as the percentage of bacteria initially coincubated with Caco2 cells (% of inoculum) (mean ± s.e.m.; n = 11; *P < 0.05; ***P < 0.001 vs. E. coli; Friedman test with Dunn’s correction). C–E, Electron microscopy observations of S. warneri internalized in Caco2 cells (Bcw: bacterial cell wall; Ex: extracellular medium; Iv: internalization vacuole; Nu: nucleus; Pm: plasma membrane; Vm: vacuolar membrane)