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Fig. 4 | Gut Pathogens

Fig. 4

From: Development and evaluation of a simple PCR assay and nested PCR for rapid detection of clarithromycin-resistant Helicobacter pylori from culture and directly from the biopsy samples in India

Fig. 4

Detection of CLR sensitive and resistant type 23S rRNA of H. pylori isolates by nested-ASP-PCR: Identification and amplification of 23S rRNA sequence of H. pylori from human gastric biopsy specimens for CLR sensitive (A) and resistant type (B). Lane 1 and 2 represent control CLR sensitive and resistant pure cultures respectively (DNA isolated by standard method), Lane 3 represents control CLR sensitive (DNA isolated by boiled temple method), lane 4–11 represent gastric biopsy specimens (DNA isolated by boiled temple method) and Lane 12 represents 100 bp Ladder

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