Fig. 4

CMTM3 promotes IL-8 production and inhibits the apoptosis of gastric epithelial cells by NEMO. a, b The expression level of IL-8 was analyzed by qRT-PCR (a) and ELISA (b). After overexpression of NEMO (pCMV-NEMO) in CMTM3 KO GES-1 cells for 24 h, cells were treated with H. pylori for 24 h. pCMV was the control vector. Data is representative of three independent experiments. c, d Representative Annexin V/propidium iodide staining results (c). After overexpression of NEMO (pCMV-NEMO) in CMTM3 KO GES-1 cells for 24 h, cells were treated with H. pylori for 24 h. pCMV was the control vector. Annexin V-positive cells were analyzed (d). Data is representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001