Table 1 Primer sequences and annealing temperatures for the detection of virulence genes by PCR
Virulence genes | Primer Sequences (5′–3′) | Amplicon (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|
esp (enterococcal surface protein) | F: AGATTTCATCTTTGATTCTTGG | 510 | 48 | [37] |
R: AATTGATTCTTTAGCATCTGG | ||||
gel E (gelatinase) | F: ACCCCGTATCATTGGTTT | 419 | 51 | [38] |
R: ACGCATTGCTTTTCCATC | ||||
cyl (cytolysin) | F: ACTCGGGGATTGATAGGC | 688 | 58 | [37] |
R: GCTGCTAAAGCTGCGCTT |