Fig. 1
Trim32 deficiency improved resistance against Lm infection. A Immunoblot analysis of inducible TRIM32 expression in THP-1 cells upon Lm infection. THP-1 was differentiated with 100 nM PMA for 2 days and replaced by RPMI media, followed by Lm infection at a MOI of 0.1 for indicated times. B Survival curves for WT and Trim32−/− mice infected with Lm. Mice (n = 10 per group) were injected i.p. with 1.0 × 105 CFU of Lm, and their survival was monitored daily. C, D Bacteria counts in the spleen and liver of mice infected i.p. with 1.0 × 106 CFUs of Lm. Bacterial counts were determined by colony plate count. Showed were the combined results of two independent experiments with a total of 8 to 10 mice per experimental group and time point. Mean value for each experimental group was shown by a bar and each symbol represents one mouse. Macroscopic examination of the spleen E and HE staining of liver F from infected Trim32−/− and WT mice, and the red circles indicated areas of erosion. Mice were injected i.p. with 1.0 × 106 CFUs of Lm, spleens and livers were inspected at 3 dpi. Data were a representative of three independent experiments. Statistical significance was calculated by means of (B) log-rank Mantel-Cox test, (C, D) two-way ANOVA followed by Dunnett’s multiple comparisons test; * P < 0.05, ** P < 0.01