Fig. 5

Trim32 deficiency increased macrophage bactericidal activity. A, B Growth of Lm in macrophages. Macrophages were seeded in 12 mm coverslips in 24-well culturing plates and infected with Lm at a MOI of 0.25 for 0.5 h. Lm media was then discarded and replaced with DMEM containing 50 μg/mL gentamicin, then the mixture was incubated for 1 h. Bacterial counts were determined for infected BMDMs (A) and PMs (B) at 0.5, 2, and 6 hpi. Shown were mean ± SEM of 3 mice per experimental group. Data were a representative of three independent experiments. C, D Representative flow cytometry plots. BMDMs were infected by Lm at a MOI of 0.1 for indicated times, and then viability (C) and iNOS expression (D) in BMDMs were analyzed by flow cytometry. E–F Statistical analysis of flow cytometry data from multiple replicates. BMDMs were infected by Lm at a MOI of 0.1 for indicated times, and then viability of BMDMs (E), iNOS expression in CD11b⁺ BMDMs (F). Data were a representative of three independent experiments. Statistical significance was calculated by means of A, B unpaired two-tailed Student’s t-test and E–F two-way ANOVA followed by Dunnett’s multiple comparisons test. * P < 0.05, *** P < 0.001