Fig. 4

Viable counts of stool samples obtained from all tested groups of immunization and challenge experiment at different time intervals. A graph representing viable CFUs of S. flexneri 2b serotype (ATCC 12022) cultivated from stool samples collected from all tested groups (negative control “non-immunized non-challenged”, positive control “non-immunized challenged with ATCC 12022”, Sf-BGV/TW80 “immunized with S. flexneri bacterial ghost vaccine treated with (TW80) then challenged with ATCC 12022”, and Sf-BGV/TX100 “immunized with S. flexneri bacterial ghost vaccine treated with (TX100) then challenged with ATCC 12022″). The X-axis represents four different time slots at which the stool samples were collected. The Y-axis represents the Log₁₀CFU/mL of ATCC 12022. Data represent mean ± standard errors of the mean (SEM). Significant difference between both treatments obtained using Two-way ANOVA data analysis, and considered significant if p < 0.0001, statistical analysis obtained using GraphPad Prism (8.0.2)