Fig. 1

The effect of pre-treatment with streptomycin and sodium bicarbonate on MAP infection. A C57BL/6 mice were given 20 mg of streptomycin or no pre-treatment followed by an oral gavage of 10⁸ CFU strep-R MAP 24-hours later. Mice were sacrificed and the large intestines were assessed for CFUs 48-hours post-infection. B Large intestine CFUs were compared between mice given streptomycin pre-treatment or no pre-treatment (*p < 0.05). C Strep-R MAP was subject to a pH of 7.0 or 3.0 for 1, 2, or 4 hours. The CFUs following incubation at either pH was plotted over time (*p < 0.05, **p < 0.01). D Mice were orally given 20 mg of streptomycin. The next day half of the mice were given sodium bicarbonate followed by a gavage of 10⁸ CFU strep-R MAP in all mice 30 minutes later. Mice were sacrificed and their organs were assessed for CFUs 48-hours post-infection. E–G The MAP CFUs of the large intestine (E), small intestine (F), and MLNs (G) were compared between mice given sodium bicarbonate and streptomycin pre-treatment or only streptomycin pre-treatment