Fig. 3

Assessment of chronic infection and disease following streptomycin pre-treatment and MAP gavage. A Mice were pre-treated with 20 mg streptomycin followed by 2 consecutive doses of 10⁹ CFU strep-R MAP given 24-hours apart. Mice were euthanized and assessed at 48-hours, 4-, 8-, 12-, and 24-weeks post-infection. B–D Strep-R MAP CFUs were assessed in the large intestine (B), MLNs (C), and small intestine (D) throughout the 24-week experiment. E Representative sections of the large intestine (H&E, original magnification x100), small intestine (x100), and MLNs (x40) from mice 12-weeks post-MAP infection are shown. F Fecal pellets were obtained from uninfected mice and strep-R MAP infected mice 4-, 8-, 12-, and 24-weeks post-infection and processed. The amount of lipocalin-2 found in samples from both groups was evaluated by ELISA