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Fig. 3 | Gut Pathogens

Fig. 3

From: The immune-adjunctive potential of recombinant LAB vector expressing murine IFNλ3 (MuIFNλ3) against Type A Influenza Virus (IAV) infection

Fig. 3

Optimization of A/PR/8/1934 (H1N1) virus infection in murine B16F10 cells. To optimize the viral infection in murine B16F10 melanoma cells, two MOIs (0.1 and 1.0) of the influenza A/PR/8/1934 (H1N1) virus were chosen. Virus infection was characterized by recording the visible CPEs characterized by rounding, swelling, and cellular detachment of virus-infected cells (b, c) compared to control cells (a). Virus-infected cells showing progressive accumulation of viral NP intracellularly (green fluorescence) (e, f, d). Images were captured in a Nikon TS100 inverted light microscope (Nikon, Tokyo, Japan) at ×20 magnification (a–c) and Leica SP8 confocal microscope using oil immersion ×63 objective; Scale Bar: 10 µm (d–f). Comparative analysis of percentage (%) of cell survival (g) and quantification of viral M-gene transcripts (h) in the cells infected with 0.1 and 1.0 MOI of the virus

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Gut Pathogens

ISSN: 1757-4749