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Fig. 4 | Gut Pathogens

Fig. 4

From: The immune-adjunctive potential of recombinant LAB vector expressing murine IFNλ3 (MuIFNλ3) against Type A Influenza Virus (IAV) infection

Fig. 4

In vitro transcriptional profile of immune-regulatory and antiviral genes in response to MuIFNλ3 pre-treatment of B16F10 cells. Gene expression profiles of MX-1, ISG-15, IRF-7, IL-4, IL-6, and IL-10 genes were assessed in rMuIFNλ3 pre-treated murine B16F10 cells through semi-quantitative PCR. The total RNA was extracted from different treatment regimens: Cells only (no treatment, medium only), WT L. lactis (empty vector/NZ9000 cells), rL. lactis (nisin-induced), rMuIFNλ3 protein, and subjected to cDNA synthesis. The murine GAPDH gene served as an internal control. The data presented mean fold changes (compared to control cells) of four independent experiments performed under similar conditions. Asterisk (*) indicates the statistically significant difference, and “ns” indicates a non-significant difference

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Gut Pathogens

ISSN: 1757-4749