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Fig. 7 | Gut Pathogens

Fig. 7

From: The immune-adjunctive potential of recombinant LAB vector expressing murine IFNλ3 (MuIFNλ3) against Type A Influenza Virus (IAV) infection

Fig. 7

Schematic of in vitro experimental setup using a transwell system. Representative assay setup in transwell system showing murine B16F10 cells grown in bottom wells and nisin-induced rL. lactis cells were added in the upper inserts. After 4 h, cells were washed and incubated further for 12 h followed by infection with the virus (1.0 MOI). Residual infectivity of the virus was determined by cell survivability assay/CPE observation, M-gene quantification, presence of viral NP, and HA assay. In addition, before virus infection, the expression of various antiviral genes and cytokines was checked

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