Table 1 List of the bacterial strains, plasmids, and viruses used in this study
Bacterial strains, plasmids and viruses | Details | Purpose | Source |
|---|---|---|---|
pHis-TEV | Ampr, 6X-Histidine tag (N-terminal), T7 promoter, TEV-tag and cleavage site | Expression vector | BioBharati LifeSciences, India |
pHis-MuIFNλ3 | Ampr, 6X-Histidine tag (N-terminal), TEV-tag and cleavage site, T7 promoter in plasmid backbone. Harbours the MuIFNλ3 gene sequence | Expression of recombinant Murine-IFNλ3 protein | This work |
pSec-MuIFNλ3 | Cmr, spUSP45, pNisA promoter, trpA terminator in plasmid backbone, harbouring the MuIFNλ3 gene sequence | Expression of recombinant Murine-IFNλ3 in L. lactis | This work |
E. coli BL21 (DE3) | F– ompT Ion hsdSB (rB–, mB–) gal dcm (DE3) | Expression host for recombinant proteins | BioBharati LifeSciences |
L. lactis (NZ9000) | MG1363 (nisRK genes into chromosome), Wild type plasmid free | Wild type bacteria (control) | Dr. Luis G Bermúdez Humar´an, French National Institute for Agricultural Research, Paris, France |
rL. lactis (rNZ9000) | MG1363 (nisRK genes into chromosome), harbouring the pSec-MuIFNλ3 plasmid | Expression host for recombinant proteins in the secretory form | This work |
Influenza A Virus (H1N1) | A/PR/8/1934(H1N1) (VR-95™) | To infect murine B16F10 cells (IBSC approval no. IISERK/IBSC/2019/005) | ATCC, USA |