Fig. 1
From: A monoclonal antibody collection for C. difficile typing ?
Generation of anti-LMW-specific hybridomas from immunized mice. a Sequence alignments of the LMW of five clinical ribotypes (LMW-RT001, LMW-RT002, LMW-RT014, LMW-RT027, LMW-RT078) by ClustalOmega software. Fully conserved residues are indicated by (*), groups of strongly similar properties by (:) and groups of weakly similar properties by (.). Signal peptide, domain 1 and 2 and the domain that interacts with the HMW are indicated. b Schematic of the generation of mice knock-in for the human variable VDJ segments in the endogenous variable heavy chain locus, and for the human variable VJ segments in the endogenous variable light chain kappa locus. c Protocol outline. Mice were immunized with LMW proteins according to the represented scheme combined to alum and Bordetella pertussis toxin. Four days after the last boost, spleens were collected and hybridoma generated. d Sera titers at day 42 of immunized mice for recombinant LMW-RT001, LMW-RT002, LMW-RT014, LMW-RT078, LMW-RT027 measured by ELISA. OD values for several dilutions for mice #1 to #5 are represented. Black curves (-) represent sera titers of a naive mouse