Fig. 4

Binding of mAbs to LMWs expressed at the surface of C. difficile bacteria. a Right: Flow cytometry analysis of mAbs binding to LMW of indicated C. difficile ribotypes. Results are displayed as staining index (refer to methods section). Left: representative histograms for staining of strain RT078 by mAbs PH4, RA11, SC6 and QE2 are shown. b Growth of C. difficile strain RT027 in BHISG medium incubated with indicated anti-LMW027 mAb or with an unspecific IgG (isotype). Growth was followed continuously over 24 h. Each dot represents the mean of three technical replicates, and the bars indicate standard deviations. ns: non-significant