Fig. 5

Antimicrobial effects of culture supernatants from vole Lactobacillus strains. Growth inhibition of C. albicans, E. coli, P. aeruginosa, and S. aureus in presence of the supernatants of the probiotic strains L. rhamnosus GG (LGG), L. johnsonii ATCC 33200 (Lj), and eleven selected strains of vole lactobacilli is depicted. Graphs depict the percent growth of the indicator microorganisms in 200 µL total culture volume following addition of 1.5, 25, 50 and 100 µL of Lactobacillus culture supernatants. Percent values were calculated from control growth, i.e., no supernatant added to the culture. Assay results are graphed for the most efficient strains in inhibiting bacterial growth. In general, antifungal activities towards C. albicans were less potent and only effective at high supernatant concentrations. Data points are mean values from three experiments with duplicate measurements. L. johnsonii ATCC 33200 (green data points) and L. rhamnosus GG (red data points) were included as reference strains