Amoxicillin modulates gut microbiota to improve short-term high-fat diet induced pathophysiology in mice

Kumar, Suresh; Raj, V. Samuel; Ahmad, Ayaan; Saini, Vikram

doi:10.1186/s13099-022-00513-0

Gut Pathogens

Table 6 Effect of diet on mice gut microbiota at genus level

From: Amoxicillin modulates gut microbiota to improve short-term high-fat diet induced pathophysiology in mice

Genus	Relative abundance at genus level (%)				Significance (adjusted p value)
Genus	CD	CD + A	HFD	HFD + A	CD vs CD + A	CD vs HFD	HFD vs HFD + A
Unc Gemellaceae	0.0 ± 0.000	0.0 ± 0.000	0.2 ± 0.044	0.01 ± 0.009	NS	< 0.0001	< 0.0001
Granulicatella	0.0 ± 0.000	0.0 ± 0.000	0.2 ± 0.044	0.1 ± 0.031	NS	< 0.0001	< 0.0001
Enterococcus	0.0 ± 0.000	0.0 ± 0.000	0.1 ± 0.031	3.0 ± 0.170	NS	NS	< 0.0001
Lactobacillus	4.5 ± 0.207	0.1 ± 0.031	15.2 ± 0.359	0.3 ± 0.054	< 0.0001	< 0.0001	< 0.0001
Streptococcus	0.0 ± 0.000	0.0 ± 0.000	0.6 ± 0.077	0.5 ± 0.070	NS	< 0.0001	0.0341
Turicibacter	0.0 ± 0.000	0.0 ± 0.000	0.5 ± 0.070	0.0 ± 0.000	NS	< 0.0001	< 0.0001
Unc Clostridiales	39.1 ± 0.489	0.6 ± 0.077	32.7 ± 0.469	11.3 ± 0.316	< 0.0001	< 0.0001	< 0.0001
Unc Clostridiaceae	0.0 ± 0.000	0.0 ± 0.000	0.0 ± 0.000	0.2 ± 0.044	NS	NS	< 0.0001
Candidatus Arthromitus	5.7 ± 0.232	0.1 ± 0.031	4.8 ± 0.213	0.1 ± 0.031	< 0.0001	< 0.0001	< 0.0001
Dehalobacterium	0.6 ± 0.077	0.0 ± 0.000	0.1 ± 0.031	0.0 ± 0.000	< 0.0001	< 0.0001	0.0077
Unc Lachnospiraceae	9.5 ± 0.293	0.3 ± 0.054	2.5 ± 0.156	12.8 ± 0.334	< 0.0001	< 0.0001	< 0.0001
Blautia	0.1 ± 0.031	0.0 ± 0.000	0.1 ± 0.031	2.0 ± 0.140	< 0.0001	NS	< 0.0001
Coprococcus	0.8 ± 0.089	0.0 ± 0.000	0.8 ± 0.089	1.6 ± 0.239	< 0.0001	NS	< 0.0001
Dorea	0.0 ± 0.000	0.0 ± 0.000	0.2 ± 0.044	0.0 ± 0.000	NS	< 0.0001	< 0.0001
Oscillospira	1.8 ± 0.132	0.1 ± 0.031	3.1 ± 0.173	0.8 ± 0.089	< 0.0001	< 0.0001	< 0.0001
Ruminococcus	1.9 ± 0.136	0.0 ± 0.000	0.6 ± 0.077	0.2 ± 0.044	< 0.0001	< 0.0001	< 0.0001
Veillonella	0.0 ± 0.000	0.0 ± 0.000	0.1 ± 0.031	0.4 ± 0.063	NS	0.0020	< 0.0001
Unc Erysipelotrichaceae	0.2 ± 0.044	0.1 ± 0.031	0.3 ± 0.054	16.6 ± 0.372	0.0019	NS	< 0.0001
Allobaculum	0.0 ± 0.000	0.0 ± 0.000	0.4 ± 0.063	0.0 ± 0.000	NS	< 0.0001	< 0.0001
Eubacterium	0.1 ± 0.031	0.1 ± 0.031	0.2 ± 0.044	16.6 ± 0.372	NS	NS	< 0.0001
Rhodobacter	0.0 ± 0.000	0.1 ± 0.031	0.3 ± 0.054	0.1 ± 0.031	0.0019	< 0.0001	< 0.0001
Sutterella	0.0 ± 0.00	2.2 ± 0.146	0.0 ± 0.000	0.2 ± 0.044	< 0.0001	NS	< 0.0001
Bilophila	0.0 + 0.000	0.0 ± 0.000	0.1 ± 0.031	0.0 ± 0.00	NS	< 0.0001	< 0.0001
Desulfovibrio	3.0 ± 0.170	0.2 ± 0.044	7.4 ± 0.261	0.2 ± 0.044	< 0.0001	< 0.0001	< 0.0001
Flexispira	0.0 ± 000	0.0 ± 0.000	0.0 ± 0.000	0.7 ± .083	NS	NS	< 0.0001
Helicobacter	0.0 ± 000	0.8 ± 0.089	0.0 ± 0.000	0.1 ± 0.031	< 0.0001	NS	< 0.0001
Unc Enterobacteriaceae	0.2 ± 0.044	9.7 ± 0.295	0.2 ± 0.044	0.6 ± 0.077	< 0.0001	NS	< 0.0001
Klebsiella	0.5 ± 0.070	37.5 ± 0.484	0.6 ± 0.077	2.7 ± 0.162	< 0.0001	NS	< 0.0001
Haemophilus	0.0 ± 000	0.0 ± 000	0.3 ± 0.054	0.1 ± 0.031	NS	< 0.0001	< 0.0001
Bacteroides	0.2 ± 0.044	26.7 ± 0.442	0.2 ± 0.044	1.4 ± 0.117	< 0.0001	NS	< 0.0001
Prevotella	0.0 ± 0.00	0.5 ± 0.070	0.1 ± 0.031	0.3 ± 0.054	< 0.0001	< 0.0001	< 0.0001
Unc S24-7	4.1 ± 0.115	4.9 ± 0.215	0.3 ± 0.054	1.4 ± 0.117	< 0.0001	< 0.0001	< 0.0001
Unc C111	0.7 ± 0.083	1.0 ± 0.099	0.4 ± 0.063	0.7 ± 0.083	0.0002	< 0.0001	< 0.0001
Unc ACK-M1	0.8 ± 0.089	1.2 ± 0.108	0.5 ± 0.070	0.7 ± 0.083	< 0.0001	< 0.0001	< 0.0001
Rothia	0.0 ± 0.000	0.0 ± 0.000	0.7 ± 0.083	0.6 ± 0.077	NS	< 0.0001	NS
Unc Coriobacteriaceae	0.1 ± 0.031	0.0 ± 0.000	2.7 ± 0.162	0.1 ± 0.031	< 0.0001	< 0.0001	< 0.0001
Adlercreutzia	1.3 ± 0.113	0.1 ± 0.031	3.9 ± 0.193	5.6 ± 0.229	< 0.0001	< 0.0001	< 0.0001
Atopobium	0.0 ± 0.000	0.0 ± 0.000	0.0 ± 0.000	0.1 ± 0.031	NS	NS	< 0.0001
Akkermansia	0.0 ± 0.000	0.10 ± 0.031	0.0 ± 0.000	0.1 ± 0.031	< 0.0001	NS	< 0.0001

Values are expressed as the mean ± SD (n = 6). Data were analyzed by One-way ANOVA followed by Bonferroni post-hoc test, p ≤ 0.05, NS= not significant, CD: Standard- chow diet for 3 weeks; CD + A: Standard- chow diet for 3 weeks and subjected to amoxicillin treatment (50 mg/kg body weight) in 3rd week; HFD: high-fat diet for 3 weeks (73% energy from fat); HFD + A: High-fat diet for 3 weeks and subjected to amoxicillin treatment (50 mg/kg body weight) in 3rd week. Unc=Unculturable

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ISSN: 1757-4749

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