- Open Access
Neutrophil activation by Campylobacter concisus
© Sørensen et al.; licensee BioMed Central Ltd. 2013
- Received: 19 April 2013
- Accepted: 28 June 2013
- Published: 3 July 2013
Campylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. The magnitude of systemic immunoglobulin response in acute infection is unknown.
Neutrophils from healthy volunteers were activated with five faecal isolates of C. concisus from patients with gastroenteritis as well as the oral reference strain C. concisus ATCC33237. Neutrophils were tested for the expression of adherence molecule CD11b by immunoflourescence and for oxidative burst response by chemiluminescence. The opsonic activity in a chemiluminescence assay was assessed with heat treated serum from patients with C. concisus infection.
A strong and dose-dependent activation of neutrophil adherence molecule CD11b and oxidative burst response was demonstrated with all six C. concisus isolates. Bacteria opsonised in heat treated serum induced an increased chemiluminescence response. Heat treated serum from patients with C. concisus infection did not have a higher opsonic activity than heat treated serum from healthy volunteers.
C. concisus has the capability to activate the innate immune system by stimulating neutrophil cells to increased adherence molecule expression and oxidative burst response, both crucial for acute inflammation. In a chemiluminescence assay the opsonic activity of heat treated serum from patients was not increased compared to heat treated control serum suggesting a weak systemic IgG response to infection.
- Campylobacter concisus
- Oxidative Burst
Mucosal inflammation associated with Campylobacter jejuni/coli enteritis is well described and in vitro examinations of C. jejuni/coli have documented activation of neutrophil cells in various assays relevant for acute inflammation [1–5]. Recently another species, Campylobacter concisus, has been reported as an emerging enteric pathogen of unclear potential for gastrointestinal disease. In patients with prolonged diarrhoea  as well as inflammatory bowel disease [7–9]C. concisus is associated with clinical manifestations of mucosal inflammation. However, the mechanisms of action are unknown and the magnitude of host inflammatory response to the pathogen is not well described. Our group previously documented that both faecal and oral isolates of C. concisus exhibit the potential to induce impairment of the intestinal barrier function by inducing apoptotic leaks . Furthermore C. concisus isolates have been shown to have the ability to attach to and invade human intestinal epithelial cells and induce cytokine production . Proteins of C. concisus from Crohn’s disease patients have been identified to be immunoreactive  but further knowledge of the human immune reaction towards C. concisus is limited.
Neutrophil cells are crucial part of the innate immune system and responsible for immediate local inflammatory responses. Initial and early response of the peripheral blood neutrophil upon stimulation with soluble substances including cytokines is the up-regulation of adherence molecules. When neutrophils subsequently interact with microorganisms, either by phagocytosis or by binding soluble pathogen products at the membrane, an activation of the oxidative metabolism takes place. This is part of the host defence against the microorganisms but is at the same time responsible for tissue changes and features of inflammation. The aim of our study was to examine the capability of clinical isolates of C concisus to activate neutrophil cells for pro-inflammatory functions reflecting initial adherence to the endothelium and subsequent oxidative burst response upon activation. Furthermore, we tested the immunoglobulin-dependent opsonic capacity of serum for magnifying the neutrophil response to bacterial challenge and compared serum from patients with culture-proven C. concisus infection with serum from healthy controls.
Up-regulation of CD11b
Dose-dependency of neutrophil up-regulation of CD11b at the given ratio of C. concisus to neutrophils compared to the expression when cells were exposed to PBS
Dose-dependency of up-regulation of CD11b in neutrophils from three different donors at the given ratio of C. concisus to neutrophils compared to the expression when cells were exposed to PBS
Oxidative burst response of neutrophils opsonised with pooled serum from healthy donors
Neutrophil reaction towards different strains of C. concisus
Neutrophil activation by different isolates of Campylobacter concisus
Oxidative burst response2(n=4)
Oxidative burst response of neutrophils to C. concisus opsonised with patient sera
When measuring the oxidative burst reaction of neutrophils towards C. concisus opsonised with heat treated serum from 40 C. concisus infected patients they did not generate a larger response than healthy adult donors (data not shown). Furthermore, the opsonic activity in heat treated serum from patients with C. concisus infection remained unchanged four weeks after the baseline visit (p=0.15). The opsonic activity of heat treated serum from patients had no association with clinical characteristics (i.e. fever, bloody stools, duration of illness, or age of the patient).
Campylobacter concisus, originally associated with the human oral cavity, has been proposed as an emerging pathogen causing gastrointestinal disease in humans . C. concisus requires specific hydrogen-enriched microaerobic conditions for growth, probably causing it to be underreported, since such conditions are not frequently used in routine clinical laboratories. Furthermore, the isolation from human faecal specimens is facilitated by the filter method , which may not be in general use in diagnostic laboratories. To investigate the potential of this organism to initiate inflammatory disease, we examined the pathogen-host relationship with use of neutrophil cells of healthy donors. Firstly the expression of CD11b on the surface of phagocytes exposed to C. concisus was measured using flow cytometry. CD11b is necessary for the adhesion of neutrophils to the vascular endothelium in response to cytokines released from tissue invaded by a pathogen. Up-regulation of CD11b on the surface is one of the first signs of phagocytes responding to a pathogen. Secondly, measurements of oxidative burst response were carried out both towards C. concisus opsonised with heat-treated serum from diarrhoeic C. concisus infected patients, C. concisus opsonised with heat-treated pooled serum from healthy donors and C. concisus alone.
Clinically, C. concisus have recently been associated with prolonged diarrhoea in 80% of patients with culture-positive C. concisus gastroenteritis, whereas the pathogen gives rise to an elevated C-reactive protein level only about half as often as C. jejuni/coli (48% compared to 94%) . These findings could be consistent with an evasive property in C. concisus towards the immune system, causing the responsiveness of neutrophils to be compromised. The observation also suggests that the pathogenesis of C. concisus associated pathology differs from the acute mucosal gastroenteritis well described for C. jejuni/coli. A more superficial mucosal interaction with C. concisus is likely, and consequently less involvement of a systemic inflammatory response (C-reactive protein increase) is to be expected. In this study we found that neutrophil cells from healthy donors respond to C. concisus in a dose-dependent manner both when investigating the expression of CD11b on the cell surface and the outburst of reactive oxygen intermediates (ROIs). The extracellular release of toxic oxygen radicals from neutrophils in the acute inflammation might contribute to the local tissue damage during infection and potentiate the inflammatory reaction. Furthermore we showed that although neutrophils respond towards C. concisus without these being opsonised with serum the response is increased when bacteria are opsonised. Previous studies have found that opsonised C. jejuni generates a higher degree of neutrophil granulocyte response than non-opsonised bacteria [1, 2, 15] correlating well with our findings. Our study design does not allow us to definitely conclude whether opsonisation induces increased phagocytosis of C. concisus. Previous investigations of C. jejuni and H. pylori[2–4] have shown that opsonisation significantly increases phagocytosis in both complement-dependent and –independent assays. Combination of heat-treated and untreated serum produced a higher opsonic activity for C. jejuni stimulation of neutrophils .
Our findings did not indicate any major differences in the neutrophil stimulating properties of the six different C. concisus isolates investigated differing from the results investigating isolates of C. jejuni , where large variations was reported. In other assays of inflammatory mediators, however, heterogeneity of C concisus isolates has been reported . It is possible that various subgroups of C. concisus exist and preliminary genetic data suggest C concisus to be a very heterogeneous species. However, for the five clinical isolates and the oral reference strain we observed no substantial difference in the responses upon neutrophil stimulation. It remains to be further examined if differences in genetic characteristics are translated into differences in the activation of the innate immune system of neutrophils. Moreover, it is striking that an oral isolate exhibited the same activity as the faecal isolates. At this point it is controversial if oral and faecal strains of C. concisus are linked or are separate subgroups. Several of the isolates investigated (75775, 118452, 119100 and 1718) have in a previous study been shown to have the ability to perturb the barrier function of HT-29/B6 intestinal epithelial cells . Furthermore, the pathogenic potential of C. concisus has been demonstrated in models of cellular invasiveness in human intestinal epithelial cells . Whether or not C. concisus is able to be invasive in the host is unknown, but no report on bloodstream isolation of C. concisus has been published. C. concisus opsonised with serum from C. concisus positive patients did not generate a larger neutrophil response than those opsonised with pooled serum from healthy individuals, indicating a weak IgG response towards the bacteria. However, we cannot exclude that the sensitivity of the assay employed in the present study does not allow to detect specific IgG activity. Previously, another mucosal gastroenteric pathogen, Helicobacter pylori, was reported to be opsonised equally well with sera from infected patients and healthy controls . So far, no assay of serum immunoglobulin concentration specifically for C. concisus has been reported, so the magnitude of systemic antibody response is unclear.
Human neutrophil cells show an innate immune reaction towards C. concisus even at low concentrations at both the initiation of the immune-inflammatory response with up-regulation of the adherence molecule CD11b as well as later in the response with the outburst of reactive oxygen radicals. The latter increases when the pathogen is opsonised with serum, but we did not observe immunoglobulin associated amplification of the response in heat treated serum from patients with C. concisus infection. The findings of pro-inflammatory activation in neutrophils support the potential and clinical relevance of C. concisus as an emerging enteric pathogen. The outcome of C. concisus associated diarrhoea is very diverse and it remains to be investigated if the responses of the innate immune system correlate with the clinical presentation.
Isolates of campylobacter concisus
Five faecal isolates from diarrheic patients were selected for the study (C. concisus isolates 2010–1718, 2009–75775, 2009–118452, 2009–119100, and 2012–197540 as previously published ) as well as the oral reference strain C. concisus ATCC 33237). Isolates were cultured on 5% horse blood agar, containing 1% yeast extract (SSI Diagnostica, Hillerød, Denmark), and incubated at 37°C in a microaerobic atmosphere with 3% hydrogen. Final confirmation was obtained through a species-specific real-time PCR based on the cpn60 gene, as described by Chaban et al. . After two days bacterial cells were suspended in phosphate buffered saline (PBS) to a concentration of approximately 75 × 106 CFU/ml (absorbance 0.25 McFarland units) and transferred to 2 ml aliquots that were stored at −20°C until use.
Measurement of optical density
To ensure that the concentration of the six different C. concisus isolates were the same throughout the experiments, measurements of optical density were carried out using Spectramax (Molecular Devices, LLC, USA) at a wavelength of 600 nm and converted to concentration using a linear model made from a series of measurements of the isolate 2010–1718.
Isolation of neutrophil granulocytes
Peripheral venous blood was taken from healthy adult donors, anticoagulated by EDTA and separated by centrifugation (500 g for 30 minutes) on polymorphprep (Axis-Shield, Dundee, Scotland). Neutrophils were washed three times in PBS-AG (100 ml PBS, 0.1g albumin, 0.2 ml 50%glucose), and the final neutrophil cell concentration was adjusted to 2 × 106 cells/ml suspended in PBS-AG using sysmex xs-1000i (Sysmex Corporation, Kobe, Japan).
Serum samples were taken from 40 diarrheic patients with culture-positive C. concisus stool samples approximately 4 weeks after the diagnosis had been made . For the pooled serum used for controls, serum samples from five healthy male donors were used. Serum was transferred to cryovials and stored at −80°C until use. Before use serum samples were heat treated at 56°C for 30 minutes to inactivate the complement system and then diluted to 1:200 with PBS. In prior experiments this concentration was found to be optimal for the oxidative burst response assay (data not shown).
Adherence molecule expression assay
Oxidative burst response assay
Luminol dependent chemiluminiscence was used to measure the oxidative burst response of neutrophils against C. concisus isolate 2010–1718 opsonised with heat treated patient serum and heat treated pooled donor serum, respectively. For each serum sample a negative control with PBS was run in parallel. All samples were run as duplicates and on every plate a control without serum was run in parallel. Forty patient samples were run three times each with 2–3 patient samples per plate. Each plate contained a control with pooled serum and neutrophils from a healthy donor. In total 54 plates were run. 100 μl luminol, 25 μl heat-treated serum, 50 μl neutrophil cells (2 × 106 cells/ml) and 100 μl bacterial suspension or PBS was added to the wells of a white polysorb microtiter plate and run immediately in at 37°C using Lumistar (BMG Labtech, Ortenberg, Germany). The peak value was reached after approximately 30minutes and each experiment ran for at least 40 minutes. Peak luminescence values were corrected by subtracting the peak value of unstimulated cells.
When comparing the neutrophil reaction towards different C. concisus isolates the test was run simultaneously for all strains with neutrophils from the same donor and heat-treated pooled serum using the same quantities as mentioned above. All samples were run in duplicates. The results are presented as ratios between the peak luminescence value and the value of unstimulated cells.
Statistical analysis was carried out using probit plots and the student’s t-test for normally distributed data and the Wilcoxon-test for non-normally distributed data.
Scientific and ethics approval for the study was obtained from the Ethics Committee for North Denmark Region (N-20080056).
- Ullmann U, Krausse R: The reaction of campylobacter species on the chemiluminescence, chemotaxis and hemagglutination. Zbl Bakt Hyg A. 1987, 266: 178-190.Google Scholar
- Bär W, Glenn-Calvo E, Krausse R: Phagocytosis of enteric campylobacter by human and murine granulocytes. FEMS Microbiol Immunol. 1991, 76: 143-150. 10.1111/j.1574-6968.1991.tb04207.x.View ArticleGoogle Scholar
- Walan Å, Dahlgren C, Kihlström E, Stendahl O, Lock R: Phagocyte killing of Campylobacter jejuni in relation to oxidative metabolism. APMIS. 1992, 100: 424-430. 10.1111/j.1699-0463.1992.tb00893.x.View ArticlePubMedGoogle Scholar
- Kist M, Spiegelhalder C, Moriki T, Schaefer HE: Interaction of Helicobacter pylori (strain 151) and Campylobacter coli with human peripheral polymorphonuclear granulocytes. Zbl Bakt. 1993, 280: 58-72.View ArticleGoogle Scholar
- Murphy H, Cogan T, Humphrey T: Direction of neutrophil movements by campylobacter-infected intestinal epithelium. Microb Infect. 2011, 13: 42-48. 10.1016/j.micinf.2010.09.007.View ArticleGoogle Scholar
- Nielsen HL, Ejlertsen T, Engberg J, Nielsen H: Short-term and medium-term clinical outcomes of Campylobacter concisus infection. Clin Microbiol Infect. 2012, 18: E459-E465. 10.1111/j.1469-0691.2012.03990.x.View ArticlePubMedGoogle Scholar
- Man SM, Zhang L, Day AS, Leach ST, Lemberg DA, Mitchell H: Campylobacter concisus and other campylobacter species in children with newly diagnosed Crohn’s disease. Inflamm Bowel Dis. 2010, 16: 1008-1016. 10.1002/ibd.21157.View ArticlePubMedGoogle Scholar
- Mukhopadhya I, Thomson JM, Hansen R, Berry SH, El-Omar EM, Hold GL: Detection of Campylobacter concisus and other campylobacter species in colonic biopsies from adults with ulcerative colitis. PLoS ONE. 2011, 6: e21490-10.1371/journal.pone.0021490.PubMed CentralView ArticlePubMedGoogle Scholar
- Mahendran V, Riordan SM, Grimm MC, Tran TAT, Major J, Kaakoush NO, Mitchell H, Zhang L: Prevalence of campylobacter species in adult Crohn’s disease and the preferential colonization sites of campylobacter species in the human intestine. PLos ONE. 2011, 6: e25417-10.1371/journal.pone.0025417.PubMed CentralView ArticlePubMedGoogle Scholar
- Nielsen HL, Nielsen H, Ejlertsen T, Engberg J, Günzel D, Zeitz M, Hering NA, Fromm M, Schulzke JD, Bücker R: Oral and fecal Campylobacter concisus strains perturb barrier function by apoptosis induction in HT-29/B6 intestinal epithelial cells. PloS ONE. 2011, 6: e23858-10.1371/journal.pone.0023858.PubMed CentralView ArticlePubMedGoogle Scholar
- Man SM, Kaakoush NO, Leach ST, Nahidi L, Lu HK, Norman J, Day AS, Zhang L, Mizchell HM: Host attachment, invasion and stimulation of proinflammatory cytokines by Campylobacter concisus and other non-campylobacter jejuni campylobacter species. J Infect Dis. 2010, 202: 1855-1865. 10.1086/657316.View ArticlePubMedGoogle Scholar
- Kovach Z, Kaakoush NO, Lamb S, Zhang L, Raftery MJ, Mitchell H: Immunoreactive proteins of Campylobacter concisus, an emergent intestinal pathogen. FEMS. 2011, 63: 387-396.Google Scholar
- Man SM: The clinical importance of campylobacter species. Nature Rev Gastro Hepatol. 2011, 8: 669-685. 10.1038/nrgastro.2011.191.View ArticleGoogle Scholar
- Engberg J, On SL, Harrington CS, Gerner-Smidt P: Prevalence of campylobacter, arcobacter, helicobacter, and sutturella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. J Clin Microbiol. 2000, 38: 286-291.PubMed CentralPubMedGoogle Scholar
- Bernatowska E, Jose P, Davies H, Stephenson M, Webster D: Interaction of campylobacter species with antibody, complement and phagocytes. Gut. 1989, 30: 906-911. 10.1136/gut.30.7.906.PubMed CentralView ArticlePubMedGoogle Scholar
- Kaakoush NO, Deshpande NP, Wilkins MR, Tan CG, Burgos-Portugal JA, Raftery MJ, Day AS, Lemberg DA, Mitchell H: The pathogenic potential of Campylobacter concisus strains associated with chronic intestinal diseases. PLoS ONE. 2011, 6: e29045-10.1371/journal.pone.0029045.PubMed CentralView ArticlePubMedGoogle Scholar
- Das SS, Karim QN, Easmon CSF: Opsonophagocytosis of Campylobacter pylori. J Med Microbiol. 1988, 27: 125-130. 10.1099/00222615-27-2-125.View ArticlePubMedGoogle Scholar
- Nielsen HL, Ejlertsen T, Engberg J, Nielsen H: High incidence of Campylobacter concisus in gastroenteritis in North Jutland, Denmark: a population-based study. Clin Microbiol Infect. 2013, 19: 445-450. 10.1111/j.1469-0691.2012.03852.x.View ArticlePubMedGoogle Scholar
- Chaban B, Musil KM, Himsworth CG, Hill JE: Development of cpn60-based real-time quantitative PCR assays for the detection of 14 campylobacter species and application to screening of canine fecal samples. Appl Environ Microbiol. 2009, 75: 3055-61. 10.1128/AEM.00101-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Van Eeden SF, Klut ME, Walker BAM, Hogg JC: The use of flow cytometry to measure neutrophil function. J Immunol Meth. 1999, 232: 23-43. 10.1016/S0022-1759(99)00148-9.View ArticleGoogle Scholar
- Hansen PS, Go MF, Varming K, Andersen LP, Graham DY, Nielsen H: Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori is not associated with cagA, vacA, or picB genotypes. APMIS. 1999, 107: 1117-1123. 10.1111/j.1699-0463.1999.tb01517.x.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.