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Draft genome sequence of non-shiga toxin-producing Escherichia coli O157 NCCP15738
Gut Pathogens volume 8, Article number: 13 (2016)
The non-shiga toxin-producing Escherichia coli (non-STEC) O157 is a pathogenic strain that cause diarrhea but does not cause hemolytic-uremic syndrome, or hemorrhagic colitis. Here, we present the 5-Mb draft genome sequence of non-STEC O157 NCCP15738, which was isolated from the feces of a Korean patient with diarrhea, and describe its features and the structural basis for its genome evolution.
A total of 565-Mbp paired-end reads were generated using the Illumina-HiSeq 2000 platform. The reads were assembled into 135 scaffolds throughout the de novo assembly. The assembled genome size of NCCP15738 was 5,005,278 bp with an N50 value of 142,450 bp and 50.65 % G+C content. Using Rapid Annotation using Subsystem Technology analysis, we predicted 4780 ORFs and 31 RNA genes. The evolutionary tree was inferred from multiple sequence alignment of 45 E. coli species. The most closely related neighbor of NCCP15738 indicated by whole-genome phylogeny was E. coli UMNK88, but that indicated by multilocus sequence analysis was E. coli DH1(ME8569).
A comparison between the NCCP15738 genome and those of reference strains, E. coli K-12 substr. MG1655 and EHEC O157:H7 EDL933 by bioinformatics analyses revealed unique genes in NCCP15738 associated with lysis protein S, two-component signal transduction system, conjugation, the flagellum, nucleotide-binding proteins, and metal-ion binding proteins. Notably, NCCP15738 has a dual flagella system like that in Vibrio parahaemolyticus, Aeromonas spp., and Rhodospirillum centenum. The draft genome sequence and the results of bioinformatics analysis of NCCP15738 provide the basis for understanding the genomic evolution of this strain.
Escherichia coli is a gram-negative bacterium that colonizes the human gastrointestinal tract. Most E. coli serotypes are non-pathogenic but some serotypes cause food poisoning. E. coli strains are divided into three subgroups, according to their pathogenicity: nonpathogenic, pathogenic, and extra intestinal pathogenic E. coli. There are 190 serotypes  of E. coli, based on the major surface antigens (O, H, and K) . The serotype O157:H7 is the major strain in the enterohemorrhagic E. coli (EHEC) serotype and since 1982, these strains have been found to be important food-borne pathogens . This type of E. coli can cause hemorrhagic colitis and hemolytic uremic syndrome (HUS) [4, 5]. O157:H7 can be identified by a combination of biochemical and immunological markers, such as sorbitol  in combination with O antigen . E. coli O157:H7 is characterized by the expression of shiga-like toxins even though it produces various other virulence factors [8–10]. Shiga toxins are classified into two major groups, Stx1 and Stx2, which are encoded on a prophage . These genes can be transferred horizontally to E. coli and other Enterobacteriaceae species , allowing transformation of shiga-like toxin non-producing strains into shiga-like toxin-producing strains . The capillary endothelial cells are the major target sites of shiga toxins released by shiga toxin-producing E. coli (STEC). Specifically, the shiga toxins target the globotriaosylceramide receptor on the cells and are transported into the cells by receptor-mediated endocytosis . Shiga toxins halt protein synthesis by cleaving an adenine base from the ribosomes of the intruded cells . This blockage can cause kidney failure, as in HUS .
Although serotype STEC O157 strains are prevalent, non-STEC O157 strains have also been reported in children with diarrhea . Very little is known about the symbiosis and pathogenicity of non-STEC O157 strains in the host; therefore, their genomes should be sequenced to assess horizontal gene transfer (HGT) and to understand the evolution of these strains. In this study, we performed genomic sequencing to investigate the genetic background of the evolution of non-STEC O157 NCCP15738 isolated from a patient with diarrhea. We also performed genomic comparison between the genomes of NCCP15738 and two reference strains, E. coli K-12 substr. MG1655  and EHEC O157:H7 str. EDL933  to study their evolution and phylogenetic linkage.
Strain, isolation, and serotyping
A fecal sample from a patient with diarrhea was plated on MacConkey agar directly or, occasionally, after enrichment in trypticase soy broth containing vancomycin (Sigma Chemicals Co., St. Louis, MO). Candidate colonies were then plated on trypticase soy agar medium and biochemically characterized using the API20E system (Biomerieux, Marcy l’Etoile, France). For O-antigen determination, we used the method described by Guinee et al.  and all available O (O1–O181) antisera. All antisera were absorbed with the corresponding cross-reacting antigens to remove non-specific agglutinins. The O antisera were produced at Laboratorio de Referencia de E. coli (Lugo, Spain [http://www.lugo.usc.es/ecoli]). This research was approved by the Research Ethics Committee of the Korea Centers for Disease Control and Prevention, and written informed consent was obtained from the patient. The isolated strain was deposited at the National Culture Collection for Pathogens (NCCP) at Korea National Institute of Health under the accession number NCCP15738. E. coli K-12 substr. MG1655 and EHEC O157:H7 str. EDL933 were used as the reference strains because these strains represent non-STECs and STECs, respectively.
Library preparation and whole-genome sequencing
The genomic DNA of NCCP15738 was purified and fragmented randomly. After fragmentation, the overhangs were converted into blunt ends using T4 DNA polymerase, Klenow Fragment, and T4 Polynucleotide Kinase (New England Biolabs, MA, USA). Sequencing adapters were ligated to the ends of the end-repaired DNA fragments. The DNA fragments that met the required length were retained by gel electrophoresis and amplified by PCR. We used the Illumina-HiSeq 2000 (Illumina, San Diego, CA, USA) platform for whole-genome sequencing and produced 565,810,000 bp data with paired end reads of 90-bp length and 500-bp insert size.
Genome assembly and annotation
For quality control of the sequencing data, the following steps were employed. First, reads with more than 9 % Ns’ bases or low complexity reads were discarded. Second, reads with more than 40 bases of low quality (≤Q20) were discarded. Third, adapter sequences with at least 15 bp overlap between adapter and reads that allowed 3 bp mismatches were removed. Fourth, duplicated reads were discarded. After quality control removals, we obtained 504 Mbp of high quality reads. SOAPdenovo (version 1.05)  was used for de novo assembly of the genome using the high quality reads. For the purpose of assembly correction, all reads that passed the quality control were aligned against the assembly result using SOAPaligner (version 2.21) . The single base error of the assembly result was corrected using mapping information. Scaffolds over 500 bp in length were considered for downstream analysis. To predict open reading frames (ORFs) and annotate the ORFs, we used the RAST (Rapid Annotation using Subsystem Technology, version 4.0)  server pipeline. We compared the predicted CDSs (coding DNA sequences) of NCCP15738 to those of two E. coli strains, K-12 substr. MG1655 and E. coli O157:H7 str. EDL933, using OrthoMCL software (version 2.0.9) . Orthologous protein sequences were clustered into groups and the orthologous proteins of all three E. coli strains in each group were counted. To identify the virulence factor genes in NCCP15738, we performed a BLAST (Basic Local Alignment Search Tool) search of whole NCCP15738 ORFs against the virulence factor genes listed in VFDB  with an e-value of 1e-5. Insertion sequences (ISs) were identified by mapping to a sequence database that was downloaded from IS Finder DB (http://www-is.biotoul.fr), using RepeatMasker (version 4.0.1) (http://www.repeatmasker.org). Phage-associated gene clusters in the scaffolds of NCCP15738 were searched using the PHAST server  (data not shown).
Phylogenetic analysis and comparative genomic analysis
To infer the evolutionary history of NCCP15738, we performed a multiple sequence alignment of the whole genome using Mugsy (version 1.2.3)  and approximately-maximum-likelihood phylogenetic trees were inferred using FastTree (version 2.1.7)  with a GTR (generalized time-reversible) + CAT model . The tree was visualized using FigTree (version 1.3.1) (http://tree.bio.ed.ac.uk/software/figtree/). In order to exclude the effect of HGT in our phylogenetic analysis, we used the multilocus sequence analysis (MLSA) method [28, 29]. Seven housekeeping genes (adk, fumC, gyrB, icd, mdh, purA, and recA) from 45 E. coli strains were retrieved and concatenated. A phylogenetic tree of multi locus sequence typing (MLST) genes was created using the method employed for whole-genome phylogenetic analysis. Mauve (version 2.3.1)  was used for comparative genomics using the Move Contig tool. The scaffolds were reordered against the complete genome of the reference E. coli strain, K-12 substr. MG1655. From the comparative genomic study, we identified a syntenic region that aligned against the reference genome. Unaligned scaffolds against the reference genome were defined as unique regions of NCCP15738. We also used the progressive alignment algorithm of Mauve for comparative alignment of NCCP15738, E. coli strain K-12 substr. MG1655, and E. coli strain O157:H7 str. EDL933 genomes. The BLAST algorithm was used to identify syntenic genes between the species and to analyze the genes of interest.
The genomic DNA was purified from a pure culture of a single bacterial isolate of NCCP15738. Potential contamination of the genomic library by other microorganisms was assessed using a BLAST search against the non-redundant database. We also checked for contamination by other genomes by confirming coverage distribution.
Results and discussion
Whole-genome sequencing by Illumina-HiSeq 2000 showed 565,810,000 bp with paired end reads that were 90 bp in length. After quality control, 504 Mbp of high quality reads were kept for assembly. The average sequencing depth was 86.7-fold coverage and the coverage ratio was 84.77 %. The high quality reads were assembled into 135 scaffolds by de novo assembly with an N50 value of 142,450 bp. The predicted genome size of NCCP15738 was 5,005,278 bp with 50.65 % G+C content. RAST analysis identified 4780 putative ORFs and 31 RNA genes, of which 4181 (80.6 %) could be functionally annotated (Fig. 1). The monosaccharides (212 ORFs) and central carbohydrate metabolism (135 ORFs) subsystems were significantly abundant among the subsystems (18.7 %). According to the subsystem results, we can assume that the NCCP15738 developed systems that can utilize various monosaccharides in addition to glucose to adapt to an extreme environment. A large number of ORFs were also associated with the “Amino acids and derivatives” subsystem (395 ORFs), “cofactors, vitamins, prosthetic groups, pigments” subsystem (266 ORFs) and “cell wall and capsule” subsystem (266 ORFs).
Comparative genomics of NCCP15738 with other E. coli strains
The phylogenetic comparison of gene candidates predicted by SEED  revealed E. coli O104:H4 GOS1  as the closest neighbor of NCCP15738 (score 513). To investigate the detailed evolutionary history of NCCP15738, we performed a multiple sequence alignment of 45 E. coli species including NCCP15738 (Fig. 2, Additional file 1: Table S1). Whole-genome phylogenetic analysis revealed that NCCP15738 did not cluster with E. coli strain K-12 substr. MG1655 into a single clade. Moreover, NCCP15738 was not grouped with E. coli O157:H7 str. EDL933. The most closely related neighbor of NCCP15738 was the pathogenic E. coli UMNK88 . In MLSA, NCCP15738 clustered with E. coli DH1 (ME8569) into a single clade. The E. coli UMNK88 strain and K-12 substr. MG1655 were farther from NCCP15738 in the MLSA tree than in the whole-genome phylogenetic tree. However, this difference between the whole-genome phylogenetic tree and the MLST phylogenetic tree was not significant, as there was consensus in the topology among trees. It is concordant with previous research with Phylomark .
Comparison of functional genes
A comparison of NCCP15738 genes and those of the two reference strains, E. coli K-12 substr. MG1655 and E. coli O157:H7 str. EDL933 showed that most of the functional genes of NCCP15738 were conserved in the two reference strains, but 941 genes were unique (Additional file 2: Table S2). Unique genes in NCCP15738 included those encoding lysis protein S, a two-component signal transduction system, conjugation, the flagellum, nucleotide-binding proteins, and metal ion binding proteins, explain the phenotypic differences that result from environmental adaptation. In particular, NCCP15738 has a dual flagella system used for swarming in viscous media. This system resembles those found in Vibrio parahaemolyticus, Aeromonas spp., and Rhodospirillum centenum . Sixty-five genes encoded the flagellar biosynthesis protein or the flagellar structural protein. Seven of the flagella-related proteins (1–6, and 9) were highly conserved in V. parahaemolyticus and in nine other strains (Fig. 3, Additional file 3: Table S3). Lateral flagella have no effect on pathogenicity, but the polar flagellum is important in the pathogenesis of V. parahaemolyticus . Therefore, we can suppose that the polar flagellum of NCCP15738 is the major machinery for swarming and has a pathogenic effect. In contrast, the lateral flagellum of NCCP15738 is likely related only to locomotion in this strain.
Even though NCCP15738 belongs to serotype O157, it causes diarrhea but not HUS in human hosts. Because of this, we were particularly interested in identifying the potential virulence factors within the genome of NCCP15738. The features that we identified through sequence analysis are detailed in Table 1, which includes a variety of pilus and fimbriae genes and their associated operons. However, NCCP15738 produces no shiga toxins, such as Stx1 (stx1A, stx1B) or Stx2 (stx2A, stx2B), and has only one locus of enterocyte effacement (LEE) that encodes type three secretion system (TTSS) (escR) . From our comparison of NCCP15738 with E. coli K-12 substr. MG1655 and E. coli O157:H7 str. EDL933, we found that NCCP15738 has only one unique virulence gene, papD. NCCP15738 has 19 virulence genes and 18 of these genes had been previously reported in the other two strains.
This study shows a broad comparative genomics approach to the study of the NCCP15738 genome and describes the features of this type of non-STEC O157. This information will be useful for studying the evolution of the pathogenic mechanisms in this strain and its adaptation to the environment.
Availability of supporting data
Nucleotide sequence accession numbers: This Whole Genome Shotgun project has been deposited in DDBJ/EMBL/GenBank under the accession number ASHB00000000.
coding DNA sequences
enterohemorrhagic Escherichia coli
horizontal gene transfer
multilocus sequence analysis
multi locus sequence typing
National Culture Collection for Pathogens
open reading frames
Rapid Annotation Using Subsystem Technology
shiga toxin-producing Escherichia coli
Stenutz R, Weintraub A, Widmalm G. The structures of Escherichia coli O-polysaccharide antigens. FEMS Microbiol Rev. 2006;30(3):382–403.
Orskov I, Orskov F, Jann B, Jann K. Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977;41(3):667–710.
Riley LW, Remis RS, Helgerson SD, McGee HB, Wells JG, Davis BR, Hebert RJ, Olcott ES, Johnson LM, Hargrett NT, et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983;308(12):681–5.
Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998;11(1):142–201.
Caprioli A, Morabito S, Brugere H, Oswald E. Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Vet Res. 2005;36(3):289–311.
Ratnam S, March SB, Ahmed R, Bezanson GS, Kasatiya S. Characterization of Escherichia coli serotype O157:H7. J Clin Microbiol. 1988;26(10):2006–12.
Guinee PA, Agterberg CM, Jansen WH. Escherichia coli O antigen typing by means of a mechanized microtechnique. Appl Microbiol. 1972;24(1):127–31.
Griffin PM, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome. Epidemiol Rev. 1991;13:60–98.
Johannes L, Romer W. Shiga toxins–from cell biology to biomedical applications. Nat Rev Microbiol. 2010;8(2):105–16.
Suh JK, Hovde CJ, Robertus JD. Shiga toxin attacks bacterial ribosomes as effectively as eucaryotic ribosomes. Biochemistry. 1998;37(26):9394–8.
Friedman DI, Court DL. Bacteriophage lambda: alive and well and still doing its thing. Curr Opin Microbiol. 2001;4(2):201–7.
Beutin L. Emerging enterohaemorrhagic Escherichia coli, causes and effects of the rise of a human pathogen. J Vet Med B Infect Dis Vet Public Health. 2006;53(7):299–305.
O’Brien AD, Newland JW, Miller SF, Holmes RK, Smith HW, Formal SB. Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science. 1984;226(4675):694–6.
Karmali MA. Infection by Shiga toxin-producing Escherichia coli: an overview. Mol Biotechnol. 2004;26(2):117–22.
Sandvig K, Bergan J, Dyve AB, Skotland T, Torgersen ML. Endocytosis and retrograde transport of Shiga toxin. Toxicon. 2010;56(7):1181–5.
Blank TE, Lacher DW, Scaletsky IC, Zhong H, Whittam TS, Donnenberg MS. Enteropathogenic Escherichia coli O157 strains from Brazil. Emerg Infect Dis. 2003;9(1):113–5.
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, et al. The complete genome sequence of Escherichia coli K-12. Science. 1997;277(5331):1453–62.
Perna NT, Plunkett G 3rd, Burland V, Mau B, Glasner JD, Rose DJ, Mayhew GF, Evans PS, Gregor J, Kirkpatrick HA, et al. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature. 2001;409(6819):529–33.
Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, Li Y, Li S, Shan G, Kristiansen K, et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res. 2010;20(2):265–72.
Li R, Yu C, Li Y, Lam TW, Yiu SM, Kristiansen K, Wang J. SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics. 2009;25(15):1966–7.
Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008;9:75.
Li L, Stoeckert CJ Jr, Roos DS. OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Res. 2003;13(9):2178–89.
Chen L, Yang J, Yu J, Yao Z, Sun L, Shen Y, Jin Q. VFDB: a reference database for bacterial virulence factors. Nucleic Acids Res. 2005;33(Database issue):D325–8.
Zhou Y, Liang Y, Lynch KH, Dennis JJ, Wishart DS. PHAST: a fast phage search tool. Nucleic Acids Res. 2011;39(Web Server issue):W347–52.
Angiuoli SV, Salzberg SL. Mugsy: fast multiple alignment of closely related whole genomes. Bioinformatics. 2011;27(3):334–42.
Price MN, Dehal PS, Arkin AP. FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol. 2009;26(7):1641–50.
Stamatakis A. Phylogenetic models of rate heterogeneity: a high performance computing perspective. Parallel and Distributed Processing Symposium, 2006 IPDPS 2006 20th International 2006.
Khan NH, Ahsan M, Yoshizawa S, Hosoya S, Yokota A, Kogure K. Multilocus sequence typing and phylogenetic analyses of Pseudomonas aeruginosa Isolates from the ocean. Appl Environ Microbiol. 2008;74(20):6194–205.
Glaeser SP, Kampfer P. Multilocus sequence analysis (MLSA) in prokaryotic taxonomy. Syst Appl Microbiol. 2015;38(4):237–45.
Darling AC, Mau B, Blattner FR, Perna NT. Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res. 2004;14(7):1394–403.
Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ, Disz T, Edwards RA, Gerdes S, Parrello B, Shukla M, et al. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res. 2014;42(Database issue):D206–14.
Brzuszkiewicz E, Thurmer A, Schuldes J, Leimbach A, Liesegang H, Meyer FD, Boelter J, Petersen H, Gottschalk G, Daniel R. Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC). Arch Microbiol. 2011;193(12):883–91.
Shepard SM, Danzeisen JL, Isaacson RE, Seemann T, Achtman M, Johnson TJ. Genome sequences and phylogenetic analysis of K88- and F18-positive porcine enterotoxigenic Escherichia coli. J Bacteriol. 2012;194(2):395–405.
Sahl JW, Matalka MN, Rasko DA. Phylomark, a tool to identify conserved phylogenetic markers from whole-genome alignments. Appl Environ Microbiol. 2012;78(14):4884–92.
McCarter LL. Dual flagellar systems enable motility under different circumstances. J Mol Microbiol Biotechnol. 2004;7(1–2):18–29.
Lee H-G, Jeong B-G, Park K-S. Role of Dual Flagella in the Pathogenesis of Vibrio parahaemolyticus. Fisherries Aqua Sci. 2011;14(2):73–8.
SHC and WK planned and directed the project, and interpreted the results. SHC drafted the manuscript. KSK, YBY, and YSB interpreted the results. JBK characterized the strain and prepared the genomic DNA. TK performed the gene annotation, comparative genomic analysis and wrote the manuscript. All authors read and approved the final manuscript.
This work was supported by a Grant from Marine Biotechnology Program (Genome Analysis of Marine Organisms and Development of Functional Applications) funded by the Ministry of Oceans and Fisheries and the Korea National Institute of Health (NIH 4800-4845-300 and NIH 4800-4847-300 to S.H.C.).
The authors declare that they have no competing interests.
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Kwon, T., Kim, J., Bak, Y. et al. Draft genome sequence of non-shiga toxin-producing Escherichia coli O157 NCCP15738. Gut Pathog 8, 13 (2016). https://doi.org/10.1186/s13099-016-0096-2
- Non-shiga toxin-producing Escherichia coli O157
- Draft genome
- Dual flagella