Whole-genome sequence assembly of Pediococcus pentosaceus LI05 (CGMCC 7049) from the human gastrointestinal tract and comparative analysis with representative sequences from three food-borne strains
© lv et al.; licensee BioMed Central Ltd. 2014
Received: 19 June 2014
Accepted: 18 August 2014
Published: 30 August 2014
Strains of Pediococcus pentosaceus from food and the human gastrointestinal tract have been widely identified, and some have been reported to reduce inflammation, encephalopathy, obesity and fatty liver in animals. In this study, we sequenced the whole genome of P. pentosaceus LI05 (CGMCC 7049), which was isolated from the fecal samples of healthy volunteers, and determined its ability to reduce acute liver injury. No other genomic information for gut-borne P. pentosaceus is currently available in the public domain.
We obtained the draft genome of P. pentosaceus LI05, which was 1,751,578 bp in size and possessed a mean G?+?C content of 37.3%. This genome encoded an abundance of proteins that were protective against acids, bile salts, heat, oxidative stresses, enterocin A, arsenate and universal stresses. Important adhesion proteins were also encoded by the genome. Additionally, P. pentosaceus LI05 genes encoded proteins associated with the biosynthesis of not only three antimicrobials, including prebacteriocin, lysin and colicin V, but also vitamins and functional amino acids, such as riboflavin, folate, biotin, thiamine and gamma-aminobutyrate. A comparison of P. pentosaceus LI05 with all known genomes of food-borne P. pentosaceus strains (ATCC 25745, SL4 and IE-3) revealed that it possessed four novel exopolysaccharide biosynthesis proteins, additional putative environmental stress tolerance proteins and phage-related proteins.
This work demonstrated the probiotic properties of P. pentosaceus LI05 from the gut and the three other food-borne P. pentosaceus strains through genomic analyses. We have revealed the major genomic differences between these strains, providing a framework for understanding the probiotic effects of strain LI05, which exhibits unique physiological and metabolic properties.
The genus Pediococcus belongs to the family Lactobacillaceae in the order Lactobacillales. Currently, it is comprised of eleven valid published species, including Pediococcus acidilactici, P. stilesii, P. pentosaceus, P. siamensis, P. cellicola, P. argentinicus, P. parvulus, P. ethanolidurans, P. claussenii, P. inopinatus and P. damnosus. The majority of the members of the genus Pediococcus are used in the food and drink industry as starter and probiotic cultures as well as food spoilers . P. pentosaceus has been intensively investigated and widely employed for food preservation due to its ability to produce antimicrobial agents . Additionally, several strains of P. pentosaceus have been shown to reduce inflammation, encephalopathy , obesity and fatty liver  in animals. Although food is the main source of P. pentosaceus for humans, the strains of P. pentosaceus adapted to the gastrointestinal tract are dissimilar from those found in food because the former may originate from sub-populations present in food at low numbers that exhibit special adaptive properties .
Previously, we have isolated a potential probiotic, P. pentosaceus LI05 (CGMCC 7049), from the fecal samples of healthy volunteers. This strain is tolerant to bile and acid and possesses strong antimicrobial activities against tested enteropathogens. More importantly, the administration of P. pentosaceus LI05 during acute D-galactosamine-induced liver injury in rats was shown to reduce elevated alanine aminotransferase and aspartate aminotransferase levels, prevent the increase of total bilirubin, reduce the histological abnormalities of both the liver and terminal ileum, decrease bacterial translocation, increase the serum levels of IL-10 and result in a cecal microbiome that differ from that of the liver injury control .
In this study, we present a summary, classification and the unique characteristics of human gut-borne P. pentosaceus LI05 in addition to a high-quality draft genome sequence and annotations. The probiotic properties of P. pentosaceus LI05 were analyzed using these genomic sequences combined with data from our previous study. Because the genome sequences of P. pentosaceus SL4 from kimchi , P. pentosaceus IE-3 from a dairy effluent sample , and P. pentosaceus ATCC25745 from plant  are now available, this research will provide an essential resource for elucidating the differences between strains isolated from food and the human gastrointestinal tract.
Determination of cultural, morphological and physiological properties
Growth was investigated under different temperature, pH and NaCl conditions. Cell morphologies, motilities and sporulation activities were examined using transmission electron (H-600, Hitachi Ltd., Tokyo, Japan) microscopy. Phenotypic identification was achieved with API CH50 strips and the API CHL medium system according to the manufacturers instructions (BioMrieux SA, Marcy-lEtoile, France). Other physiological and biochemical tests were conducted as described previously . Phylogenetic analysis was conducted using the neighbor-joining method based on the 16S rRNA and housekeeping gene sequences .
Cultural conditions and DNA isolation
After revival using standard methods, the P. pentosaceus LI05 strain (CGMCC 7049) was anaerobically cultured in DeMan-Rogosa-Sharpe (MRS; OXOID, Thermo Fisher Biochemicals Ltd., Beijing, China) broth at 37C for 24 h. Cells were obtained by centrifugation at 8,000 g for 10 min at 4°C. DNA was extracted using the QIAamp DNA Micro Kit according to manufacturer’s guidelines (Qiagen, Westburgb.v., Leusden, The Netherlands).
Genome sequencing and assembly
The genome of P. pentosaceus LI05 was sequenced with the next-generation sequencing platform Illumina HiSeq 2000, and the total number of reads based on a 500-bp library database were 2×11,079,017 (bp). The quality of the sequencing read data was estimated by calculating the quality and GC content of each read. The draft genome sequence was assembled using SOAPdenovo2 , and iterative optimization was used to obtain the optimal k-mer value through the use of 31-85 k-mers. The 500-bp libraries were used to build scaffolds, and the SOAPdenovo gap closer software was also used (http://soap.genomics.org.cn/soapdenovo.html). To close the remaining gaps, reference-guided assemblies were carried out with the CLC Genomics Workbench v. 6.05 (CLC bio, Aarbus, Denmark). The combination of de novo assembly and reference-guided assembly was performed manually using the microbial genome-finishing module in the CLC genomics workbench (CLC bio, Aarbus, Denmark). The complete genome sequence of P. pentosaceus ATCC 25745 was used as the reference genome.
P. pentosaceus LI05 genes were identified using Glimmer  together with comparative gene prediction by the direct mapping of the ORFs of the P. pentosaceus ATCC reference strain from the NCBI Genome Database. After a round of manual curation, the unannotated predicted coding sequences (CDS) were translated into amino acid sequences for a query using the NCBI non-redundant database as well as the UniProt, Pfam, COG, and InterPro databases to identify the closest existing homology annotations. Transfer RNA (tRNA) genes were detected using tRNAScanSE . Ribosomal RNAs (rRNAs) were identified using a BLASTn  search against the ribosomal RNA databases. Signal peptides were predicted using SignalP 4.0 , whereas transmembrane helices in proteins were predicted using TMHMM . The Integrated Microbial Genomes (IMG) platform (http://img.jgi.doe.gov/) was used to support additional gene prediction analyses and manual functional annotations .
A comparative genomic analysis using BRIG  was conducted comparing P. pentosaceus LI05 from the human gastrointestinal tract with three food-borne strains with available genomic sequences, including P. pentosaceus ATCC 25745, SL4 and IE-3. The P. pentosaceu s LI05 genome sequences sharing low identities (<50%) with the other strains were designated as the P. pentosaceus LI05-unique regions. The proteins encoded by the genes that only existed in P. pentosaceus LI05 or that possessed sequence similarities of less than 50% with the three food-borne strains were further analyzed by BLASTp.
Results and discussion
Classification and unique features
P. pentosaceus LI05 is a Gram-negative, non-motile, acid-tolerant, non-sporulating, spherical, facultative anaerobe from the human gastrointestinal tract (Additional file 1: Figure S1). It tolerates 6% NaCl in MRS broth. Growth occurs at 15-45°C and at pH 4-8 but optimally at 37°C. The colonies on the MRS agar were white, smooth, shiny, and circular with complete edges. Some carbohydrates, such as L-arabinose, D-ribose, D-xylose, D-galactose, D-glucose, D-fructose, D-mannose, N-acetylglucosamine, amygdalin, arbutin, salicin, D-cellobiose, D-maltose, D-trehalose, gentiobiose, and D-fucose, can be used as the sole carbon sources, whereas glycerol, erythritol, etc. cannot (Additional file 2: Table S1).
Genomic nucleotide content and gene counts
% of totala
G?+?C content (bp)
Coding region (bp)
Genes assigned to COGs
Genes with signal peptides
Genes with transmembrane helices
Genome of P. pentosaceus LI05 exhibits probiotic properties
Comparison of important genes encoding stress resistance proteins in P. pentosaceus LI05, P. pentosaceus ATCC 25745, P. pentosaceus SL4 and P. pentosaceus IE-3
Max. identity to proteins of other species (BLASTp) (%)
ATCC 25745 (%)
Choloylglycine hydrolase family protein
F0F1 ATP synthase subunit alpha
F0F1 ATP synthase subunit epsilon
F0F1 ATP synthase subunit beta
F0F1 ATP synthase subunit gamma
F0F1 ATP synthase subunit B
F0F1 ATP synthase subunit C
F0F1 ATP synthase subunit A
Universal stress resistance
Universal stress protein UspA
Universal stress protein UspA
Universal stress protein UspA
Universal stress protein UspA
Universal stress protein UspA
Universal stress protein UspA
Hyperosmotic and heat resistance
Molecular chaperone DnaJ
Environmental stress resistance
Molecular chaperone GroEL
Oxidative stress resistance
Methionine sulfoxide reductase A
Enterocin A resistance
Enterocin A Immunity family protein
Comparison of important genes encoding beneficial proteins in P. pentosaceus LI05, P. pentosaceus ATCC 25745, P. pentosaceus SL4 and P. pentosaceus IE-3
Max. identity to proteins of other species (BLASTp) (%)
ATCC 25745 (%)
Competence protein ComGC
Elongation factor Tu
Pilus biosynthesis protein HicB
Colicin V production family protein
Biosynthesis of peptidoglycans
Peptidoglycan-binding protein LysM
Riboflavin synthase, beta subunit
Riboflavin synthase, alpha subunit
Riboflavin biosynthesis protein RibD
Riboflavin biosynthesis protein RibF
Riboflavin biosynthesis acetyltransferase (GNAT) family
Biotin biosynthesis protein BioY
Thiamine biosynthesis protein ThiI
Thiamine biosynthesis protein ApbE
The P. pentosaceus LI05 genes also encoded three antimicrobials, which is consistent with the excellent antimicrobial ability of this strain. As shown in Table 3, genes encoding prebacteriocin were annotated in the genomes of both P. pentosaceus LI05 and P. pentosaceus ATCC 25745. Alternatively, the pedA gene (PCPN_1274) encoding pediocin PA-1 was detected in P. pentosaceus IE-3, but it was not identical to the prebacteriocin gene of P. pentosaceus LI05. Furthermore, genes encoding colicin V, which is a peptide antibiotic that kills sensitive cells by disrupting their membrane potentials , were found in these four P. pentosaceus strains. However, the colicin V discovered in strain L105 was different from that of the other spicies. Additionally, genes encoding lysin were detected in P. pentosaceus LI05 and P. pentosaceus ATCC 25745. As an antimicrobial agent, lysin is potentially immunogenic . Therefore, P. pentosaceus LI05 can achieve “competitive exclusion” not only by limiting the surface area available but also by secreting antimicrobial substances.
In the genome of P. pentosaceus LI05, we also detected potentially beneficial properties that were not experimentally confirmed. This strain contained genes involved in the biosynthesis of not only important vitamins, such as riboflavin, folate, thiamine and biotin but also of functional factors, such as gamma-aminobutyrate (Table 3) . In Gram-positive bacteria, peptidoglycan is one of the most important host immune regulators . Although the genes and coding proteins related to the peptidoglycan pathway were conserved in the four strains of P. pentosaceus, they were not significantly similar to those of the other species. These findings will contribute to the elucidation of the mechanisms of immune regulation in P. pentosaceus LI05.
Comparisons with other fully sequenced genomes
Genes and their encoded proteins detected in P. pentosaceus LI05 with sequence similarities of less than 50% with sequences from both P. pentosaceus ATCC 25745 and P. pentosaceus SL4
Best BLASTp hit
% Query cover
% Amino acid identity
Type I restriction endonuclease subunit S
Type I restriction-modification system, specificity subunit S
Daunorubicin resistance ATP-binding protein DrrA
Lactobacillus sanfranciscensis TMW 1.1304
ThiJ/PfpI family protein
TraX family protein
Lactobacillus buchneri CD034
Pediococcusclaussenii ATCC BAA-344
Daunorubicin resistance protein DrrC
Ferritin-like DNA-binding protein
Lactobacillus plantarum 2025
Glycosyltransferase family 2
ABC transporter permease
TetR family transcriptional regulator
Excinuclease ABC subunit A
Pediococcus acidilactic i
Acetyl xylan esterase
Enterococcus saccharol yticus
PadR family transcriptional regulator
Capsular polysaccharide biosynthesis protein
Polysaccharide biosynthesis protein
Lactobacillus plantarum WCFS1
Phage integrase family site-specific recombinase
Pediococcus acidilactici DSM 20284
Putative prophage repressor
Lactobacillus plantarum JDM1
Minor capsid protein
Minor capsid protein
Five putative exopolysaccharide biosynthesis proteins were detected only in P. pentosaceus LI05, including an epimerase, a capsular polysaccharide biosynthesis protein, two glycosyltransferases (key enzymes for the biosyntheses of the exopolysaccharide repeating units) and a polysaccharide biosynthesis protein. Four of these enzymes need to be examined in further detail because they are not only potentially novel but also probably induce variations in the structures of their encoded polysaccharides that may have influenced adherence, biofilm formation and the nature of the immune response .
P. pentosaceus LI05 was characterized by three extra-environmental stress tolerance proteins, including a putative ferritin-like DNA-binding protein, which maintains a steady state of iron ions and responds to stresses, such as those involving temperature, humidity, and ionizing and redox processes , a putative PadR family transcriptional regulator, which functions against phenolic acid stress, and a putative ThiJ/PfpI family protein, which is involved in cellular protection against environmental stresses .
Fourteen proteins related to the intrusion of exogenous DNA were identified in P. pentosaceus LI05. One group was comprised of twelve prophage-related proteins, including a phage integrase family site-specific recombinase, two integrases, a putative prophage repressor, two phage proteins, a replisome organizer, a terminase, two minor capsid proteins, a capsid protein and a tail protein. It is not rare for bacteria to contain multiple prophages in their chromosomes, which then constitute a sizable proportion of their total chromosomal material . Pathogenic, commensal, and symbiotic bacteria have been observed to play roles in a variety of bacterial adaptations in hosts . Phage-related proteins were encoded by genes in each of the three food-borne strains. The other genes detected in the P. pentosaceus LI05 included two encoding bacterial DNA type I restriction endonucleases, which are involved in prokaryotic DNA restriction-modification mechanisms that protect the bacteria against invading foreign DNA .
Two putative doxorubicin-daunorubicin resistance proteins existed in P. pentosaceus LI05. One ORF encoded DrrA, which is part of the ABC transporter complex DrrAB. The other ORF encoded DrrC, which is part of the ABC transporter permease protein. This finding partially reflects the complex interactions between drugs and gut-associated microbes . Both daunorubicin and doxorubicin are antitumor drugs and are thus not suitable for antibacterial applications. Therefore, these two genes will not affect the control of P. pentosaceus LI05.
Additionally, there were eight extra putative multifunctional proteins in P. pentosaceus LI05. These included a TetR family transcriptional regulator, an ABC transporter permease, an exonuclease ABC subunit A, a transposase, an acetyl xylan esterase, a PadR family transcriptional regulator, a membrane protein and a TraX family protein.
Strains of P. pentosaceus are frequently identified in food and in the human gastrointestinal tract and are known to reduce inflammation, encephalopathy, obesity and fatty liver in animals. Therefore, it is imperative to study the probiotic ability of this organism. Future studies will focus on delineating the interactions between the host and P. pentosaceus. The genome sequences of P. pentosaceus LI05 isolated from the human gastrointestinal tract allow for a deeper understanding of its probiotic abilities, facilitating the future development of drugs for microbiota-related diseases.
Availability of supporting data
The whole-genome sequencing project of P. pentosaceus LI05 has been submitted to GenBank under the project accession number PRJNA237570. The project version entailing the draft assembly described herein has been deposited under the accession number JDVW00000000.
L-JL designed the study, interpreted the results and edited the manuscript. L-XL and Y-DL conducted the Illumina sequencing, performed the assemblies, analyzed the genome, and performed the annotations. X-JH provided advice related to the outbreak and strain features, characterized the strain and maintained it in pure cultures. H-YS contributed to the microbiology of the strain and prepared high-molecular-weight DNA for the genome sequencing. All authors read and approved the manuscript prior to submission.
This study was supported by the National Basic Research Program of China (973 Program) (No. 2013CB531401) and the Key Program of the National Natural Science Foundation of China (No. 81330011).
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